Scholarlink

Objective <p>This study aimed to retrospectively analyze <i>Salmonella</i> Thompson isolates collected at our research center and to investigate the genetic characteristics of <i>Salmonella</i> Thompson by integrating isolates from the present study with publicly available global genomic data, with a particular focus on the ST26 clone.</p> Methods <p>This retrospective genomic epidemiological study investigated <i>Salmonella</i> Thompson isolates collected from a tertiary hospital in northern China between 2018 and 2023. We employed antimicrobial susceptibility testing, short-read and long-read whole-genome sequencing, phylogenomic analysis, and plasmid structure characterization. All <i>Salmonella</i> Thompson genomes from the NCBI GenBank database were included in comparative genomic analysis.</p> Results <p>All <i>Salmonella</i> Thompson strains in this study belonged to ST26. Strain WFS122 carried <i>bla</i><sub>NDM−5</sub>, which was located on a common IncX3-type plasmid found in NDM-positive <i>Escherichia coli</i> and <i>Klebsiella pneumoniae</i> strains (100% homology). Analysis of the public database revealed that ST26 is the globally predominant lineage of <i>Salmonella</i> Thompson, accounting for 89.57% of strains and harboring the overwhelming majority of resistance genes and Class 1 integrons. We further identified Clade D4 as a high-risk evolutionary branch with elevated resistance rates. Within this study, 96.15% of Clade D4 strains harbored Class 1 integrons, which were pivotal in resistance gene carriage, primarily encoding the aminoglycoside resistance gene <i>aadA</i> and the trimethoprim resistance gene <i>dfrA</i>.</p> Conclusion <p>We hypothesize that ST26 <i>Salmonella</i> Thompson exhibits global expansion, with the high-risk clone Clade D4 showing a strong association with Class 1 integrons and resistance genes. This high-risk clone has demonstrated potential for transmission in China, the United States, and the United Kingdom, necessitating expanded research scope and scale. Additionally, this study represents the identification of NDM-5-carrying ST26 <i>Salmonella</i> Thompson. The emergence of carbapenem-resistant <i>Salmonella</i> Thompson poses a significant challenge for clinical management of <i>Salmonella</i> infections caused by this strain.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Emergence of blaNDM−5 and international dissemination of an ST26 Salmonella Thompson resistance gene-enriched sublineage

  • 期刊论文

  • 出版模式：

    开放获取

  • 发表日期：

    2026年2月24日

查看全文
  • Haiyan Qi,
  • Chunxin Xu,
  • Xifeng Sun,
  • Xiaowei Sun

摘要

Objective

This study aimed to retrospectively analyze Salmonella Thompson isolates collected at our research center and to investigate the genetic characteristics of Salmonella Thompson by integrating isolates from the present study with publicly available global genomic data, with a particular focus on the ST26 clone.

Methods

This retrospective genomic epidemiological study investigated Salmonella Thompson isolates collected from a tertiary hospital in northern China between 2018 and 2023. We employed antimicrobial susceptibility testing, short-read and long-read whole-genome sequencing, phylogenomic analysis, and plasmid structure characterization. All Salmonella Thompson genomes from the NCBI GenBank database were included in comparative genomic analysis.

Results

All Salmonella Thompson strains in this study belonged to ST26. Strain WFS122 carried blaNDM−5, which was located on a common IncX3-type plasmid found in NDM-positive Escherichia coli and Klebsiella pneumoniae strains (100% homology). Analysis of the public database revealed that ST26 is the globally predominant lineage of Salmonella Thompson, accounting for 89.57% of strains and harboring the overwhelming majority of resistance genes and Class 1 integrons. We further identified Clade D4 as a high-risk evolutionary branch with elevated resistance rates. Within this study, 96.15% of Clade D4 strains harbored Class 1 integrons, which were pivotal in resistance gene carriage, primarily encoding the aminoglycoside resistance gene aadA and the trimethoprim resistance gene dfrA.

Conclusion

We hypothesize that ST26 Salmonella Thompson exhibits global expansion, with the high-risk clone Clade D4 showing a strong association with Class 1 integrons and resistance genes. This high-risk clone has demonstrated potential for transmission in China, the United States, and the United Kingdom, necessitating expanded research scope and scale. Additionally, this study represents the identification of NDM-5-carrying ST26 Salmonella Thompson. The emergence of carbapenem-resistant Salmonella Thompson poses a significant challenge for clinical management of Salmonella infections caused by this strain.