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<p>This study evaluated the effects of Lactic acid bacteria and cellulase, individually and in combination, on fermentation quality and microbial community dynamics of alfalfa silage. Six treatments were tested, including control, cellulase alone and two lactic acid bacteria species (<i>Pediococcus pentosaceus</i>,<i> Levilactobacillus brevis</i>) applied individually or in combination with cellulase. The results showed that <i>Levilactobacillus brevis</i> in combination with cellulase, producing higher lactic acid concentrations, lower pH (&lt; 4.2) to the other treatments. The microbiome analysis revealed that <i>Lactiplantibacillus</i> was dominant, while undesirable bacterium <i>Achromobacter</i> was suppressed. Functional prediction of microbial communities analysis indicated a higher predicted abundance of sequences associated with pyruvate metabolism, glycolysis/gluconeogenesis and starch and sucrose metabolism pathways. These findings provide insights into optimizing alfalfa silage quality through synergistic use of cellulase and lactic acid bacteria silage inoculants with high metabolic stability.</p> Graphical Abstract <p></p>

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Synergistic effects of cellulase and lactic acid bacteria(Pediococcus pentosaceus and Levilactobacillus brevis) on alfalfa silage fermentation and microbial dynamics

  • 期刊论文

  • 出版模式：

    开放获取

  • 发表日期：

    2026年3月21日

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  • Weiqin Ma,
  • Baochao Bai,
  • Mingjian Liu,
  • Panjie Sheng,
  • Jian Bao,
  • Chaoran Song,
  • Jianliang Liu,
  • Hongwei Ma,
  • Shuai Du,
  • Gentu Ge,
  • Yushan Jia,
  • Zhijun Wang

摘要

This study evaluated the effects of Lactic acid bacteria and cellulase, individually and in combination, on fermentation quality and microbial community dynamics of alfalfa silage. Six treatments were tested, including control, cellulase alone and two lactic acid bacteria species (Pediococcus pentosaceus, Levilactobacillus brevis) applied individually or in combination with cellulase. The results showed that Levilactobacillus brevis in combination with cellulase, producing higher lactic acid concentrations, lower pH (< 4.2) to the other treatments. The microbiome analysis revealed that Lactiplantibacillus was dominant, while undesirable bacterium Achromobacter was suppressed. Functional prediction of microbial communities analysis indicated a higher predicted abundance of sequences associated with pyruvate metabolism, glycolysis/gluconeogenesis and starch and sucrose metabolism pathways. These findings provide insights into optimizing alfalfa silage quality through synergistic use of cellulase and lactic acid bacteria silage inoculants with high metabolic stability.

Graphical Abstract