Background As Bacillus cereus species can negatively affect the safety and quality of both food and non-food products, it would be useful to describe among the selected strains traits supporting survival in specific matrices. We recently characterized the genome of 3 A ES, a B. cereus isolated from eye shadow, revealing multiple genes predicted to support the adaptation of this strain to harsh environmental conditions. Here, we compared the genotypic and phenotypic relative sensitivity/ resistance of B. cereus 3 A ES with two additional B. cereus cosmetic isolates: an eye shadow isolate, 7 C ES, a baby wipe isolate, 1 L BW, as well as clinical strain American Type Culture Collection (ATCC) 49,063, an emetic strain from unknown origin F60006, the ATCC 14,579 reference type strain, and a non-cereus strain, B. pumilus ATCC 7061. An additional eye shadow isolate, 8 A ES, was only tested phenotypically. Results BTyper, the B. cereus subtyping tool within the FDA HFP GalaxyTrakr bioinformatic platform, separated the strains in three phylogenetic groups, with B. pumilus as an outlier. Four strains were identified as B. cereus sensu stricto (s.s.), group IV: 3 A ES, ATCC strains 14,579 and 49,063, with 7 C ES additionally identified as B. cereus s.s. biovar Thuringiensis. F60006 was recognized as B. mosaicus subsp. cereus Emeticus s.s., in group III. Isolate 1 L BW was identified as B. cytotoxicus, group VII. Analyses of these genomic sequences using the Pathosystems Resource Integration Center (BV-BRC - PATRIC) revealed genes predicting resistance for diverse antibiotic groups and biocides. B. cereus strains displayed resistance to most of the compounds tested with Vitek® 2 Compact system and phenotype microarrays™. No differences were found between the overall average growth that correlates with the respiration response between the isolates from cosmetics and non-cosmetics (p = 0.56). However, there was a significant difference (p < 0.001) in the overall sensitivity/resistance phenotypes of the strains: the non-cereus ATCC 7061 was the least resistant strain and 3 A ES, the most resistant. Conclusion Although these B. cereus strains exhibited a range of genotypic and phenotypic profiles, there were no traits distinguishing isolates from cosmetics from the isolates from non-cosmetics tested. Further, antimicrobial resistance was not only dependent on the arsenal carried by a given strain but also on the concentration of each antibiotic.

Genomic and phenotypic diversities among Bacillus cereus isolates from cosmetics

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2025年12月19日

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Nadine Yossa,
Roma Adu-Osei,
Travis Canida,
Gopal Gopinath,
Rachel Binet

摘要

Background

As Bacillus cereus species can negatively affect the safety and quality of both food and non-food products, it would be useful to describe among the selected strains traits supporting survival in specific matrices. We recently characterized the genome of 3 A ES, a B. cereus isolated from eye shadow, revealing multiple genes predicted to support the adaptation of this strain to harsh environmental conditions. Here, we compared the genotypic and phenotypic relative sensitivity/ resistance of B. cereus 3 A ES with two additional B. cereus cosmetic isolates: an eye shadow isolate, 7 C ES, a baby wipe isolate, 1 L BW, as well as clinical strain American Type Culture Collection (ATCC) 49,063, an emetic strain from unknown origin F60006, the ATCC 14,579 reference type strain, and a non-cereus strain, B. pumilus ATCC 7061. An additional eye shadow isolate, 8 A ES, was only tested phenotypically.

Results

BTyper, the B. cereus subtyping tool within the FDA HFP GalaxyTrakr bioinformatic platform, separated the strains in three phylogenetic groups, with B. pumilus as an outlier. Four strains were identified as B. cereus sensu stricto (s.s.), group IV: 3 A ES, ATCC strains 14,579 and 49,063, with 7 C ES additionally identified as B. cereus s.s. biovar Thuringiensis. F60006 was recognized as B. mosaicus subsp. cereus Emeticus s.s., in group III. Isolate 1 L BW was identified as B. cytotoxicus, group VII. Analyses of these genomic sequences using the Pathosystems Resource Integration Center (BV-BRC - PATRIC) revealed genes predicting resistance for diverse antibiotic groups and biocides. B. cereus strains displayed resistance to most of the compounds tested with Vitek^® 2 Compact system and phenotype microarrays™. No differences were found between the overall average growth that correlates with the respiration response between the isolates from cosmetics and non-cosmetics (p = 0.56). However, there was a significant difference (p < 0.001) in the overall sensitivity/resistance phenotypes of the strains: the non-cereus ATCC 7061 was the least resistant strain and 3 A ES, the most resistant.

Conclusion

Although these B. cereus strains exhibited a range of genotypic and phenotypic profiles, there were no traits distinguishing isolates from cosmetics from the isolates from non-cosmetics tested. Further, antimicrobial resistance was not only dependent on the arsenal carried by a given strain but also on the concentration of each antibiotic.