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<p>Microbial synthesis of selenium nanoparticles (SeNPs) as a fertilizer can promote the development of selenium-rich agricultural products. However, most known selenium-reduction strains exhibit a tolerance to selenite of ≤ 100 mmol/L and possess relatively low reduction efficiency. In this study, three strains capable of tolerating selenite concentrations of &gt; 300 mmol/L were screened from selenium-rich soil in Bama, Guangxi, China. Based on 16&#xa0;S rRNA gene sequence analysis, the three strains were identified as <i>Citrobacter</i> sp.BM-1, <i>Providencia</i> sp.BM-2, and <i>Brucella</i> sp.BM-3. Notably, <i>Brucella</i> sp.BM-3 represents a novel selenium-reducing bacteria. All three strains reduced SeO<sub>3</sub><sup>2−</sup> to SeNPs on the cell membrane and subsequently released these nanoparticles outside the cells, forming spherical SeNPs with a particle size of 210–221&#xa0;nm. Furthermore, qPCR analysis revealed that the selenium-reduction mechanisms in these strains primarily involve the glutathione pathway, which is catalyzed by nitrate reductase and sulfate reductase. These findings suggest that <i>Citrobacter</i> sp.BM-1, <i>Providencia</i> sp.BM-2, and <i>Brucella</i> sp.BM-3 are promising candidates for the synthesis of SeNPs.</p>

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Three bacterial strains efficiently reduce selenite to selenium nanoparticles in cell membranes

  • 期刊论文

  • 出版模式：

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  • 发表日期：

    2025年8月29日

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  • Ruixia Li,
  • Wenqiang Chen,
  • Siyuan Huang,
  • Daihua Jiang,
  • Zhengjie Zhu,
  • Chong Li,
  • Xuejiao Huang

摘要

Microbial synthesis of selenium nanoparticles (SeNPs) as a fertilizer can promote the development of selenium-rich agricultural products. However, most known selenium-reduction strains exhibit a tolerance to selenite of ≤ 100 mmol/L and possess relatively low reduction efficiency. In this study, three strains capable of tolerating selenite concentrations of > 300 mmol/L were screened from selenium-rich soil in Bama, Guangxi, China. Based on 16 S rRNA gene sequence analysis, the three strains were identified as Citrobacter sp.BM-1, Providencia sp.BM-2, and Brucella sp.BM-3. Notably, Brucella sp.BM-3 represents a novel selenium-reducing bacteria. All three strains reduced SeO32− to SeNPs on the cell membrane and subsequently released these nanoparticles outside the cells, forming spherical SeNPs with a particle size of 210–221 nm. Furthermore, qPCR analysis revealed that the selenium-reduction mechanisms in these strains primarily involve the glutathione pathway, which is catalyzed by nitrate reductase and sulfate reductase. These findings suggest that Citrobacter sp.BM-1, Providencia sp.BM-2, and Brucella sp.BM-3 are promising candidates for the synthesis of SeNPs.