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<p><i>Vibrio alginolyticus</i> is a foodborne pathogen that poses considerable threats to human health and leads to substantial economic losses in the aquaculture industry. O and K antigen are bacterial surface structures with strong plasticity and environmental adaptability, making O antigen biosynthesis gene clusters (O-AGCs) and K antigen biosynthesis gene clusters (K-AGCs) good materials for studying bacterial genetic evolution. However, no studies have carried out in-depth analyses of the O and K antigen-related gene clusters of <i>V. alginolyticus</i>. In the present study, we conducted the first genome-based serogroup study of <i>V. alginolyticus</i> and performed a comprehensive analysis of the O- and K-AGCs. We elucidated their molecular evolutionary mechanism by comparing them at the whole gene cluster structural and molecular levels. There were 14 serotypes of O-AGCs between <i>dgkA</i> and <i>gmhD</i> and 48 serotypes of K-AGCs between <i>gmhD</i> and <i>rjg</i> among <i>V. alginolyticus</i> chromosomes. Serogroups O1, O4, and O11 were most prevalent in our sample set, accounting for approximately 60% of all <i>V. alginolyticus</i> strains. The K serogroups had more extensive diversity than the O serogroups. We further identified the K serogroup-specific gene, <i>wzy</i>, corresponding to the serogroup in a one-to-one parallel relationship. The variation in K antigen, distinguished by the unique sequence of the gene <i>wzy</i>, makes an essential contribution to the diagnostic investigations of <i>V. alginolyticus</i>. Our results represent the first insight into the O and K antigen diversity of <i>V. alginolyticus</i> and underline the importance of the structural versatility and functional adaptability of O- and K-AGCs.</p>

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Integrated analysis of O/K-antigen biosynthesis diversity and molecular serotyping scheme development of Vibrio alginolyticus

  • 期刊论文

  • 出版模式：

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  • 发表日期：

    2025年8月23日

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  • Keyi Yu,
  • Zhenzhou Huang,
  • Yanjun Li,
  • Lizhi Ma,
  • Xuemei Bai,
  • Xiuping Fu,
  • Biao Kan,
  • He Gao

摘要

Vibrio alginolyticus is a foodborne pathogen that poses considerable threats to human health and leads to substantial economic losses in the aquaculture industry. O and K antigen are bacterial surface structures with strong plasticity and environmental adaptability, making O antigen biosynthesis gene clusters (O-AGCs) and K antigen biosynthesis gene clusters (K-AGCs) good materials for studying bacterial genetic evolution. However, no studies have carried out in-depth analyses of the O and K antigen-related gene clusters of V. alginolyticus. In the present study, we conducted the first genome-based serogroup study of V. alginolyticus and performed a comprehensive analysis of the O- and K-AGCs. We elucidated their molecular evolutionary mechanism by comparing them at the whole gene cluster structural and molecular levels. There were 14 serotypes of O-AGCs between dgkA and gmhD and 48 serotypes of K-AGCs between gmhD and rjg among V. alginolyticus chromosomes. Serogroups O1, O4, and O11 were most prevalent in our sample set, accounting for approximately 60% of all V. alginolyticus strains. The K serogroups had more extensive diversity than the O serogroups. We further identified the K serogroup-specific gene, wzy, corresponding to the serogroup in a one-to-one parallel relationship. The variation in K antigen, distinguished by the unique sequence of the gene wzy, makes an essential contribution to the diagnostic investigations of V. alginolyticus. Our results represent the first insight into the O and K antigen diversity of V. alginolyticus and underline the importance of the structural versatility and functional adaptability of O- and K-AGCs.