Scholarlink

<p>As prolific producers of extracellular lytic enzymes and antimicrobial natural products, <i>Lysobacter</i> strains have garnered great interest over the past decade. Three gram-stain-negative, aerobic, short rod bacterial strains with flagella, designated A3-1-A15<sup>T</sup>, D1-1-M9<sup>T</sup> and D1-1-M8, were isolated from the shore soil of Longmucuo Lake in the Xizang Autonomous Region, China. Comparisons of the 16&#xa0;S rRNA gene sequences confirmed their affiliation with the genus <i>Novilysobacter</i>. Strain A3-1-A15<sup>T</sup> presented the highest similarity of the 16&#xa0;S rRNA gene sequence to <i>Novilysobacter selenitireducen</i> 13A<sup>T</sup> (97.75%), whereas strains D1-1-M9<sup>T</sup> and D1-1-M8 were most closely related to <i>Novilysobacter erysipheiresistens</i> RS-LYSO-3<sup>T</sup>, showing 97.80% similarity. The genomic DNA G + C contents of strains A3-1-A15<sup>T</sup>, D1-1-M9<sup>T</sup> and D1-1-M8 were 67.46%, 68.79% and 67.85%, respectively, based on the genome sequence data. The ANI values of strains A3-1-A15<sup>T</sup> and D1-1-M9<sup>T</sup> with <i>N. selenitireducens</i> A13<sup>T</sup> and <i>N. erysipheiresistens</i> CICC 23,922<sup>T</sup> were 84.45%/78.03% and 79.41%/79.81%, respectively. Corresponding dDDH values were 49.7%/23.2% and 24.7%/25.4%, all below the species thresholds, confirming two novel <i>Novilysobacter</i> species. The main polar lipids included diphosphatidylglycerol, phosphatidylethanolamine and phosphatidylglycerol. The major cellular fatty acids were summed feature 9 (C<sub>16:0</sub> 10-methyl and/or iso-C<sub>17:1</sub><i>ω</i>9<i>c</i>), iso-C<sub>15:0</sub>, iso-C<sub>16:0</sub>, iso-C<sub>11:0</sub> and iso-C<sub>11:0</sub> 3-OH. Protease production was confirmed through genomic data and physiological experiments. In summary, these findings support the proposal of two novel species within the genus <i>Novilysobacter</i>: <i>N. viscosus</i> sp. nov. and <i>N. longmucuonensis</i> sp. nov. The type strains for <i>N. viscosus</i> sp. nov. and <i>N. longmucuonensis</i> are A3-1-A15<sup>T</sup> (= CGMCC 1.19188<sup>T</sup> = KCTC 92144<sup>T</sup>) and D1-1-M9<sup>T</sup> (= CGMCC 1.19230<sup>T</sup> = KCTC 92146<sup>T</sup>), respectively.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Novilysobacter viscosus sp. nov. and Novilysobacter longmucuonensis sp. nov., two novel species capable of producing proteases and RiPPs

  • 期刊论文

  • 出版模式：

    开放获取

  • 发表日期：

    2025年7月22日

查看全文
  • Dorji Phurbu,
  • Xuan Zhang,
  • Rui Wang,
  • Zi-Xuan Liu,
  • Yan-Yan Zheng,
  • Ai-Hua Li

摘要

As prolific producers of extracellular lytic enzymes and antimicrobial natural products, Lysobacter strains have garnered great interest over the past decade. Three gram-stain-negative, aerobic, short rod bacterial strains with flagella, designated A3-1-A15T, D1-1-M9T and D1-1-M8, were isolated from the shore soil of Longmucuo Lake in the Xizang Autonomous Region, China. Comparisons of the 16 S rRNA gene sequences confirmed their affiliation with the genus Novilysobacter. Strain A3-1-A15T presented the highest similarity of the 16 S rRNA gene sequence to Novilysobacter selenitireducen 13AT (97.75%), whereas strains D1-1-M9T and D1-1-M8 were most closely related to Novilysobacter erysipheiresistens RS-LYSO-3T, showing 97.80% similarity. The genomic DNA G + C contents of strains A3-1-A15T, D1-1-M9T and D1-1-M8 were 67.46%, 68.79% and 67.85%, respectively, based on the genome sequence data. The ANI values of strains A3-1-A15T and D1-1-M9T with N. selenitireducens A13T and N. erysipheiresistens CICC 23,922T were 84.45%/78.03% and 79.41%/79.81%, respectively. Corresponding dDDH values were 49.7%/23.2% and 24.7%/25.4%, all below the species thresholds, confirming two novel Novilysobacter species. The main polar lipids included diphosphatidylglycerol, phosphatidylethanolamine and phosphatidylglycerol. The major cellular fatty acids were summed feature 9 (C16:0 10-methyl and/or iso-C17:1ω9c), iso-C15:0, iso-C16:0, iso-C11:0 and iso-C11:0 3-OH. Protease production was confirmed through genomic data and physiological experiments. In summary, these findings support the proposal of two novel species within the genus Novilysobacter: N. viscosus sp. nov. and N. longmucuonensis sp. nov. The type strains for N. viscosus sp. nov. and N. longmucuonensis are A3-1-A15T (= CGMCC 1.19188T = KCTC 92144T) and D1-1-M9T (= CGMCC 1.19230T = KCTC 92146T), respectively.