Scholarlink

Background <p>Actinomycetes of the genus <i>Streptomyces</i> are renowned for their highly developed and diverse specialized metaboliс pathways, and there is an extensive body of data on their specific and pleiotropic levels of regulation. Much less is known about routes leading to essential metabolites in this genus. In this work, we focused on elucidating the function of the highly conserved across <i>Streptomyces</i> gene <i>SCO1417</i> for GntR type regulator in the model strain <i>S. coelicolor</i> A3(2).</p> Results <p>Combining the results of knockout and promoter probe experiments, we show that the gene <i>SCO1417</i> controls pyridoxal 5ʹ-phosphate (PLP; vitamin B6) biosynthesis, and thus is a member of the PdxR group of the transcriptional regulators. The Sco1417 protein is a transcriptional repressor of its gene and an activator of the expression of the PLP synthase genes, <i>SCO1523</i> (<i>pdxS)</i> and <i>SCO1522</i> (<i>pdxT</i>). According to electrophoretic mobility shift assays, out of several tested B6 vitamers, only PLP served as a Sco1417 effector molecule. We also provide data on the location of the Sco1417 binding site within the promoter region of <i>pdxST</i>.</p> Conclusions <p>Our work portrays for the first time an evidence-based picture of the genetic control of vitamin B6 biosynthesis in <i>S. coelicolor</i> M145. Given the high conservancy and synteny of <i>pdx</i> homologs in the other streptomycetes, we suggest that the described genetic circuit is a general feature for the entire genus.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Gene sco1417 encodes a positive regulator of the de novo biosynthesis of pyridoxal 5ʹ-phosphate (vitamin B6) in Streptomyces coelicolor M145

  • 期刊论文

  • 出版模式：

    开放获取

  • 发表日期：

    2025年7月2日

查看全文
  • Olga Tsypik,
  • Roman Makitrynskyy,
  • Elisabeth Gauger,
  • Agnieszka Mitulski,
  • Andreas Bechthold,
  • Klas Flärdh,
  • Wolfgang Wohlleben,
  • Bohdan Ostash

摘要

Background

Actinomycetes of the genus Streptomyces are renowned for their highly developed and diverse specialized metaboliс pathways, and there is an extensive body of data on their specific and pleiotropic levels of regulation. Much less is known about routes leading to essential metabolites in this genus. In this work, we focused on elucidating the function of the highly conserved across Streptomyces gene SCO1417 for GntR type regulator in the model strain S. coelicolor A3(2).

Results

Combining the results of knockout and promoter probe experiments, we show that the gene SCO1417 controls pyridoxal 5ʹ-phosphate (PLP; vitamin B6) biosynthesis, and thus is a member of the PdxR group of the transcriptional regulators. The Sco1417 protein is a transcriptional repressor of its gene and an activator of the expression of the PLP synthase genes, SCO1523 (pdxS) and SCO1522 (pdxT). According to electrophoretic mobility shift assays, out of several tested B6 vitamers, only PLP served as a Sco1417 effector molecule. We also provide data on the location of the Sco1417 binding site within the promoter region of pdxST.

Conclusions

Our work portrays for the first time an evidence-based picture of the genetic control of vitamin B6 biosynthesis in S. coelicolor M145. Given the high conservancy and synteny of pdx homologs in the other streptomycetes, we suggest that the described genetic circuit is a general feature for the entire genus.