<p><i>Acinetobacter baumannii</i>, a gram-negative bacterium commonly associated with nosocomial infections, has been relatively unexplored in the environmental context. The present study was conducted in Dhaka City, Bangladesh, with the primary objective of isolating and characterizing <i>A. baumannii</i> in environmental water sources. Surface water samples were collected from various water reservoirs to detect the presence of <i>A. baumannii.</i> Isolates were confirmed as <i>A. baumannii</i> using culture and PCR. Confirmed isolates were screened for antimicrobial susceptibility, antimicrobial resistance genes, and serum resistance. Results revealed that 32% of the water samples tested positive for <i>A. baumannii</i>. In total, 23 <i>A. baumannii</i> isolates were obtained. All isolates showed resistance to Cefepime. Varying degrees of resistance to other antibiotics were observed, and 56% showed resistance to the bactericidal effect of serum. This study underscored the remarkable adaptability of <i>A. baumannii</i> and its ability to flourish in diverse environmental conditions, highlighting public health concerns of increasing antibiotic resistant bacteria. The study concluded that, given the significance of effective infection control and sanitation and waste management measures, understanding the presence and behavior of <i>A. baumannii</i> in the environment is paramount. This study acts as the first report on environmental <i>A. baumannii</i> in Bangladesh and further research is warranted to elucidate the underlying mechanisms of antibiotic resistance and their implications for human health.</p>