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Background <p>Plant diseases significantly and persistently impair product quality and yield across the globe. Employing antagonistic microorganisms represents an environmentally friendly and cost-effective approach to pathogen management. In this study, <i>Bacillus velezensis</i> Lzh-5 was explored to understand the molecular underpinnings of its antagonistic activity and plant growth-promoting properties.</p> Results <p>We present the basic genomic profile of <i>B. velezensis</i> Lzh-5. Whole-genome analysis revealed that Lzh-5 possesses a 4,015,817&#xa0;bp circular chromosome with a GC content of 46.0%, and an 8,933&#xa0;bp circular plasmid with a GC content of 40.5%. A total of 3,998 genes were predicted, of which 3,881 (97.07%) are coding DNA sequences (CDSs). Through phylogenomic and comparative genomic analyses, strain Lzh-5 was confirmed as <i>B. velezensis</i>. The Lzh-5 genome harbors genes for cell wall-degrading enzymes. Additionally, 13 gene clusters responsible for secondary metabolite production were identified. Notably, a unique cluster (cluster 2) coding for an unknown compound was found exclusively in strain Lzh-5. Genes associated with plant growth enhancement, such as those involved in chemotaxis, motility, biofilm formation, phytohormone production, nitrogen fixation, phosphate solubilization, glycine betaine biosynthesis, and acetoin and 2,3-butanediol synthesis, were also identified.</p> Conclusion <p>The basic characteristics of strain Lzh-5 genome were delineated through whole-genome sequencing. Our analysis indicates that the Lzh-5 genome encompasses various genes that promote plant growth, induce systemic resistance, and antagonize pathogens. Compared to other strains, several unique gene clusters in Lzh-5 may contribute to the discovery of novel bioactive compounds and offer a broader antagonistic spectrum. This investigation elucidates the antifungal and plant growth-promoting mechanisms of <i>B</i>. <i>velezensis</i> Lzh-5 at a genetic level, providing a theoretical foundation for further application in agricultural production.</p>

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Complete genome sequence and comparative analysis of Bacillus velezensis Lzh-5, a fungal antagonistic and plant growth-promoting strain

  • 期刊论文

  • 出版模式：

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  • 发表日期：

    2025年4月22日

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  • Dongying Zhao,
  • Yutong Wu,
  • Jie Qu,
  • Lei Fang,
  • Chaoyue Liu,
  • Lin Zhang,
  • Mingshuo Zhang,
  • Jihua Wang,
  • Zhenghua Li

摘要

Background

Plant diseases significantly and persistently impair product quality and yield across the globe. Employing antagonistic microorganisms represents an environmentally friendly and cost-effective approach to pathogen management. In this study, Bacillus velezensis Lzh-5 was explored to understand the molecular underpinnings of its antagonistic activity and plant growth-promoting properties.

Results

We present the basic genomic profile of B. velezensis Lzh-5. Whole-genome analysis revealed that Lzh-5 possesses a 4,015,817 bp circular chromosome with a GC content of 46.0%, and an 8,933 bp circular plasmid with a GC content of 40.5%. A total of 3,998 genes were predicted, of which 3,881 (97.07%) are coding DNA sequences (CDSs). Through phylogenomic and comparative genomic analyses, strain Lzh-5 was confirmed as B. velezensis. The Lzh-5 genome harbors genes for cell wall-degrading enzymes. Additionally, 13 gene clusters responsible for secondary metabolite production were identified. Notably, a unique cluster (cluster 2) coding for an unknown compound was found exclusively in strain Lzh-5. Genes associated with plant growth enhancement, such as those involved in chemotaxis, motility, biofilm formation, phytohormone production, nitrogen fixation, phosphate solubilization, glycine betaine biosynthesis, and acetoin and 2,3-butanediol synthesis, were also identified.

Conclusion

The basic characteristics of strain Lzh-5 genome were delineated through whole-genome sequencing. Our analysis indicates that the Lzh-5 genome encompasses various genes that promote plant growth, induce systemic resistance, and antagonize pathogens. Compared to other strains, several unique gene clusters in Lzh-5 may contribute to the discovery of novel bioactive compounds and offer a broader antagonistic spectrum. This investigation elucidates the antifungal and plant growth-promoting mechanisms of B. velezensis Lzh-5 at a genetic level, providing a theoretical foundation for further application in agricultural production.