Scholarlink

Background <p>Tissue-derived small extracellular vesicles (sEVs) are vital mediators of intercellular communication; however, the molecular landscape of their long RNA cargo, including full-length mRNAs and long noncoding RNAs, and the mechanisms controlling specific RNA sorting into sEVs are still poorly understood. Here, we successfully isolated sEVs from five anatomical regions of healthy porcine lung and performed polyadenylated transcriptome profiling using short- and long-read sequencing.</p> Results <p>We identified a diverse repertoire of long RNAs in lung-derived sEVs, with high concordance in transcript quantification between sequencing platforms and substantial functional conservation across anatomical regions. Notably, approximately 30% of sEV-associated long RNAs were full-length transcripts, comprising 8,374 unique isoforms. Comparative transcriptomic analyses revealed pronounced differences between lung sEVs and their parental tissues. Specifically, sEV-enriched long RNAs (557) were characterized by low-GC and repetitive motifs, in contrast to the GC-rich motifs predominantly observed in sEV-depleted transcripts (1,076). We identified that KHDRBS1 is a candidate RNA-binding protein (RBP) associated with sEV mRNA enrichment. KHDRBS1 knockdown specifically impaired the sorting of 21 sEV-enriched mRNAs that contain predicted KHDRBS1 binding sites. Additionally, epithelial and immune cells were established as the predominant contributors to lung sEVs.</p> Conclusions <p>This study provides a comprehensive transcriptional landscape of porcine lung sEVs. Our results present evidence that KHDRBS1 represents a critical candidate RNA-binding protein (RBP) associated with sEV mRNA enrichment of porcine lung.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

A comprehensive long RNA landscape of multi-regional porcine lung-derived small extracellular vesicles

  • 期刊论文

  • 出版模式：

    开放获取

  • 发表日期：

    2026年7月2日

查看全文
  • Jinxiu Liu,
  • Naixiang Yu,
  • Jiacheng Wei,
  • Zhou Zhang

摘要

Background

Tissue-derived small extracellular vesicles (sEVs) are vital mediators of intercellular communication; however, the molecular landscape of their long RNA cargo, including full-length mRNAs and long noncoding RNAs, and the mechanisms controlling specific RNA sorting into sEVs are still poorly understood. Here, we successfully isolated sEVs from five anatomical regions of healthy porcine lung and performed polyadenylated transcriptome profiling using short- and long-read sequencing.

Results

We identified a diverse repertoire of long RNAs in lung-derived sEVs, with high concordance in transcript quantification between sequencing platforms and substantial functional conservation across anatomical regions. Notably, approximately 30% of sEV-associated long RNAs were full-length transcripts, comprising 8,374 unique isoforms. Comparative transcriptomic analyses revealed pronounced differences between lung sEVs and their parental tissues. Specifically, sEV-enriched long RNAs (557) were characterized by low-GC and repetitive motifs, in contrast to the GC-rich motifs predominantly observed in sEV-depleted transcripts (1,076). We identified that KHDRBS1 is a candidate RNA-binding protein (RBP) associated with sEV mRNA enrichment. KHDRBS1 knockdown specifically impaired the sorting of 21 sEV-enriched mRNAs that contain predicted KHDRBS1 binding sites. Additionally, epithelial and immune cells were established as the predominant contributors to lung sEVs.

Conclusions

This study provides a comprehensive transcriptional landscape of porcine lung sEVs. Our results present evidence that KHDRBS1 represents a critical candidate RNA-binding protein (RBP) associated with sEV mRNA enrichment of porcine lung.