Scholarlink

Background <p>The placenta is a complex organ with multiple immune and non-immune cell types that promote fetal tolerance and facilitate the transfer of nutrients and oxygen. The nonhuman primate (NHP) is a key experimental model for studying human pregnancy complications, in part due to similarities in placental structure, which makes it essential to understand how single-cell populations compare across the human and NHP maternal–fetal interface. We constructed a single-cell RNA-Seq (scRNA-Seq) atlas of the placenta from the pigtail macaque (<i>Macaca nemestrina</i>) in the third trimester, comprising three different tissues at the maternal–fetal interface: the chorionic villi (placental disc), chorioamniotic membranes, and the maternal decidua. Each tissue was separately dissociated into single cells and processed through the 10X Genomics and Seurat pipeline, followed by aggregation, unsupervised clustering, and cluster annotation. Next, we determined the maternal–fetal origins of cell populations and analyzed single-cell RNA trajectory, Gene Ontology enrichment, and cell–cell communication.</p> Results <p>Single-cell populations in the pigtail macaque were strikingly similar in their identity and frequency to those found in the human placenta, including cells from trophoblast, stromal cell, immune, and macrophage lineages. An advantage of our approach was the deep sequencing of three tissues at the maternal–fetal interface, which yielded a rich diversity of common and rare single-cell populations.</p> Conclusions <p>Single-cell populations from the third-trimester pigtail macaque placenta are highly concordant with those from the human placenta. This scRNA-Seq atlas provides a powerful resource for understanding experimental perturbations on the NHP placenta.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

A single-cell transcriptomic atlas of the pigtail macaque placenta in late gestation

  • 期刊论文

  • 出版模式：

    开放获取

  • 发表日期：

    2026年6月26日

查看全文
  • Amanda Li,
  • Richard Li,
  • Hazel Huang,
  • Hong Zhao,
  • Briana Del Rosario,
  • Miranda Li,
  • Edmunda Li,
  • Andrew Vo,
  • Gygeria Manuel,
  • Orlando Cervantes,
  • Raj P. Kapur,
  • Jeff Munson,
  • Austyn Orvis,
  • Michelle Coleman,
  • Melissa Berg,
  • Britni Curtis,
  • Brenna Menz,
  • Jin Dai,
  • Inah Golez,
  • Solomon Wangari,
  • Chris English,
  • Audrey Baldessari,
  • Lakshmi Rajagopal,
  • John Cornelius,
  • Kristina M. Adams Waldorf

摘要

Background

The placenta is a complex organ with multiple immune and non-immune cell types that promote fetal tolerance and facilitate the transfer of nutrients and oxygen. The nonhuman primate (NHP) is a key experimental model for studying human pregnancy complications, in part due to similarities in placental structure, which makes it essential to understand how single-cell populations compare across the human and NHP maternal–fetal interface. We constructed a single-cell RNA-Seq (scRNA-Seq) atlas of the placenta from the pigtail macaque (Macaca nemestrina) in the third trimester, comprising three different tissues at the maternal–fetal interface: the chorionic villi (placental disc), chorioamniotic membranes, and the maternal decidua. Each tissue was separately dissociated into single cells and processed through the 10X Genomics and Seurat pipeline, followed by aggregation, unsupervised clustering, and cluster annotation. Next, we determined the maternal–fetal origins of cell populations and analyzed single-cell RNA trajectory, Gene Ontology enrichment, and cell–cell communication.

Results

Single-cell populations in the pigtail macaque were strikingly similar in their identity and frequency to those found in the human placenta, including cells from trophoblast, stromal cell, immune, and macrophage lineages. An advantage of our approach was the deep sequencing of three tissues at the maternal–fetal interface, which yielded a rich diversity of common and rare single-cell populations.

Conclusions

Single-cell populations from the third-trimester pigtail macaque placenta are highly concordant with those from the human placenta. This scRNA-Seq atlas provides a powerful resource for understanding experimental perturbations on the NHP placenta.