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Background <p>Plant growth and developmental processes are tightly regulated by small secreted peptides, however, the functions and mechanisms of Tyrosine Sulfation-containing Peptides (<i>PSY</i>) remain unclear. In chickpea, knowledge of <i>PSY</i> genes family is limited.</p> Results <p>This study employed comprehensive bioinformatics approaches to identify and characterize seven <i>CaPSY</i> genes in the chickpea genome. The analyses encompass chromosomal localization, evolutionary relationships, gene structure, conserved motif identification, promoter architecture, prediction of <i>PSY</i>-targeting miRNAs, and expression profiling. Chromosomal mapping revealed that <i>CaPSY</i> genes are confined to four specific chromosomes rather than being evenly distributed across the genome. Phylogenetic analysis resolved nine distinct groups, each further subdivided into subgroups. Additionally, <i>CaPSY</i> genes were found to contain one to two introns. Amino acid sequence comparisons demonstrated that each <i>CaPSY</i> gene consistently harbors a PSY domain in its C-terminal end. Promoter analysis of <i>CaPSY</i> genes revealed the presence of multiple hormone-responsive elements, including ABRE, SARE, AuxRE, and MeJARE, as well as stress-related elements such as the drought-responsive MBS, suggesting potential regulatory roles in development and stress adaptation. Further, the expression patterns of <i>CaPSY</i> were evaluated in multiple tissues as well as in response to abiotic stresses. The results indicated differential expression of <i>CaPSY</i> genes among tissues and under multiple abiotic stress conditions. We further detected several miRNAs likely to target <i>CaPSY</i> genes and assessed how they are expressed in different tissues.</p> Conclusion <p>Thus, these findings serve as a crucial resource for basic and applied research, enabling advancements in chickpea productivity and stress tolerance via precise genome editing and innovative breeding methods.</p>

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Genome-wide survey and expression analysis of peptides containing tyrosine sulfation (PSY) gene family in Cicer arietinum L.

  • 期刊论文

  • 出版模式：

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  • 发表日期：

    2026年2月21日

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  • Mahipal Singh Kesawat,
  • Vinay Kumar,
  • Swati Manohar,
  • Aqib Sohail,
  • Manjusha Rani,
  • Sang-Min Chung,
  • Deepak Kumar,
  • Sangram K. Lenka,
  • Fred Bwayo Masika

摘要

Background

Plant growth and developmental processes are tightly regulated by small secreted peptides, however, the functions and mechanisms of Tyrosine Sulfation-containing Peptides (PSY) remain unclear. In chickpea, knowledge of PSY genes family is limited.

Results

This study employed comprehensive bioinformatics approaches to identify and characterize seven CaPSY genes in the chickpea genome. The analyses encompass chromosomal localization, evolutionary relationships, gene structure, conserved motif identification, promoter architecture, prediction of PSY-targeting miRNAs, and expression profiling. Chromosomal mapping revealed that CaPSY genes are confined to four specific chromosomes rather than being evenly distributed across the genome. Phylogenetic analysis resolved nine distinct groups, each further subdivided into subgroups. Additionally, CaPSY genes were found to contain one to two introns. Amino acid sequence comparisons demonstrated that each CaPSY gene consistently harbors a PSY domain in its C-terminal end. Promoter analysis of CaPSY genes revealed the presence of multiple hormone-responsive elements, including ABRE, SARE, AuxRE, and MeJARE, as well as stress-related elements such as the drought-responsive MBS, suggesting potential regulatory roles in development and stress adaptation. Further, the expression patterns of CaPSY were evaluated in multiple tissues as well as in response to abiotic stresses. The results indicated differential expression of CaPSY genes among tissues and under multiple abiotic stress conditions. We further detected several miRNAs likely to target CaPSY genes and assessed how they are expressed in different tissues.

Conclusion

Thus, these findings serve as a crucial resource for basic and applied research, enabling advancements in chickpea productivity and stress tolerance via precise genome editing and innovative breeding methods.