Scholarlink

Background <p><i>Anoplocephala perfoliata</i> is the most prevalent and pathogenic tapeworm (cestode) of horses worldwide, yet it remains molecularly understudied. Here, we present the mitochondrial and chromosome-scale nuclear genomes and matched somatic proteome for this parasite, establishing the first high-resolution molecular resource for the family Anoplocephalidae.</p> Results <p>This parasite was first characterised morphologically and then by its mitochondrial genome (size: 13,776&#xa0;bp). Its complete nuclear genome (size: 372.3&#xa0;Mb) was assembled and characterised; it encodes 9,711 protein-coding genes, 78.2% of which were functionally annotated and ~ 80% supported by transcriptomic evidence. Proteomic analysis confirmed 758 proteins in previously-analysed excretory/secretory (ES) products from adult worms, including highly expressed components of the ubiquitin–proteasome system, stress response families – e.g., translationally controlled tumour proteins (TCTPs) and universal stress proteins (USPs) – and cytoskeletal scaffolds. Approximately 6.5% of the genome contains retroelements, predominantly LINEs. Comparative genomic analyses revealed a relatively conserved synteny with members of the family Taeniidae (<i>Echinococcus granulosus</i>, <i>E. multilocularis</i> and <i>Taenia multiceps</i>) and a pronounced structural divergence from <i>Hymenolepis microstoma</i> (Hymenolepididae), reflecting mosaic genome evolution within the order Cyclophyllidea. Classification of proteins inferred from the genome identified GTPases, kinases, peptidases and secretome-associated proteins among the most abundant groups. A subset of proteins exhibited signal peptides or extracellular localisation, suggesting their role as parasite-derived proteins (PDPs) involved in host–parasite communication and immune evasion.</p> Conclusion <p>This integrated genomic and proteomic framework reveals lineage-specific molecular adaptations in <i>A. perfoliata</i> and provides a foundation for future functional and translational investigations of this and closely related cestodes.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Chromosome-scale nuclear genome and proteome of Anoplocephala perfoliata elucidate lineage-specific features of a ‘neglected’ equine tapeworm

  • 期刊论文

  • 出版模式：

    开放获取

  • 发表日期：

    2026年1月21日

查看全文
  • Neil D. Young,
  • Tao Wang,
  • Ching-Seng Ang,
  • Dilara Lale,
  • Hans-Peter Fuehrer,
  • Sunita B. Sumanam,
  • Pasi K. Korhonen,
  • Bill C. H. Chang,
  • Robin B. Gasser

摘要

Background

Anoplocephala perfoliata is the most prevalent and pathogenic tapeworm (cestode) of horses worldwide, yet it remains molecularly understudied. Here, we present the mitochondrial and chromosome-scale nuclear genomes and matched somatic proteome for this parasite, establishing the first high-resolution molecular resource for the family Anoplocephalidae.

Results

This parasite was first characterised morphologically and then by its mitochondrial genome (size: 13,776 bp). Its complete nuclear genome (size: 372.3 Mb) was assembled and characterised; it encodes 9,711 protein-coding genes, 78.2% of which were functionally annotated and ~ 80% supported by transcriptomic evidence. Proteomic analysis confirmed 758 proteins in previously-analysed excretory/secretory (ES) products from adult worms, including highly expressed components of the ubiquitin–proteasome system, stress response families – e.g., translationally controlled tumour proteins (TCTPs) and universal stress proteins (USPs) – and cytoskeletal scaffolds. Approximately 6.5% of the genome contains retroelements, predominantly LINEs. Comparative genomic analyses revealed a relatively conserved synteny with members of the family Taeniidae (Echinococcus granulosus, E. multilocularis and Taenia multiceps) and a pronounced structural divergence from Hymenolepis microstoma (Hymenolepididae), reflecting mosaic genome evolution within the order Cyclophyllidea. Classification of proteins inferred from the genome identified GTPases, kinases, peptidases and secretome-associated proteins among the most abundant groups. A subset of proteins exhibited signal peptides or extracellular localisation, suggesting their role as parasite-derived proteins (PDPs) involved in host–parasite communication and immune evasion.

Conclusion

This integrated genomic and proteomic framework reveals lineage-specific molecular adaptations in A. perfoliata and provides a foundation for future functional and translational investigations of this and closely related cestodes.