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Background <p>To explore reference genome of <i>Dracocephalum moldavica</i> L. and provide a basis for the study of the mechanism of tilianin biosynthesis.</p> Results <p>We present telomere-to-telomere (T2T) genome assembly with the size of 255.61 Mb and a contig N50 length of 58.45 Mb. By integrating multi-omics resources, we identified two UDP- glycosyltransferases, Dm7GlcT1 and Dm7GlcT2, catalyzing the glucosylation of acacetin at 7-OH, which was the final step in tilianin biosynthetic pathway. The findings from the <i>in vitro</i> enzyme activity assay and qRT-PCR analysis further indicate that Dm7GlcT1 may be responsible for catalyzing tilianin biosynthesis in stems and flowers, whereas glucosylation mediated by Dm7GlcT2 could occur in all tissues.</p> Conclusion <p>Our work presents the first T2T genome assembly of <i>D. moldavica</i> and identified two key UDP- glycosyltransferases associated with tilianin biosynthesis, which provided a valuable reference for further metabolic network construction of tilianin biosynthesis and genetic improvement of <i>D. moldavica</i> with high tilianin product.</p>

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Multi-omics analyses of Dracocephalum moldavica L. reveal two flavonoid glycosyltransferases in tilianin biosynthesis

  • 期刊论文

  • 出版模式：

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  • 发表日期：

    2026年1月23日

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  • Qian Zhao,
  • Xiaoxue Zhang,
  • Wenfang Wang,
  • Hui Yu,
  • Zhanli Wang

摘要

Background

To explore reference genome of Dracocephalum moldavica L. and provide a basis for the study of the mechanism of tilianin biosynthesis.

Results

We present telomere-to-telomere (T2T) genome assembly with the size of 255.61 Mb and a contig N50 length of 58.45 Mb. By integrating multi-omics resources, we identified two UDP- glycosyltransferases, Dm7GlcT1 and Dm7GlcT2, catalyzing the glucosylation of acacetin at 7-OH, which was the final step in tilianin biosynthetic pathway. The findings from the in vitro enzyme activity assay and qRT-PCR analysis further indicate that Dm7GlcT1 may be responsible for catalyzing tilianin biosynthesis in stems and flowers, whereas glucosylation mediated by Dm7GlcT2 could occur in all tissues.

Conclusion

Our work presents the first T2T genome assembly of D. moldavica and identified two key UDP- glycosyltransferases associated with tilianin biosynthesis, which provided a valuable reference for further metabolic network construction of tilianin biosynthesis and genetic improvement of D. moldavica with high tilianin product.