Scholarlink

Background <p>Salt stress imposes osmotic imbalance and ion toxicity, severely impairing crop growth and development. Na⁺/H⁺ antiporters (NHXs) play a central role in salt tolerance by mediating sodium transport across cellular membranes.</p> Results <p>We conducted a genome-wide characterization of the <i>NHX</i> gene family in four hexaploid oat cultivars, identifying 126 <i>NHX</i> genes. These were grouped into 33 orthologous gene groups (OGGs), comprising 10 core and 23 dispensable OGGs. The naked oat cultivar ‘Sanfensan (SFS)’ contained the largest number of both total and core <i>NHX</i> genes. Phylogenetic analysis classified <i>AsNHX</i> genes into three classes: vacuolar (Vac-class), endosomal (Endo-class), and plasma membrane (PM-class). Ka/Ks analysis indicated strong purifying selection across most members. <i>Cis</i>-regulatory element analysis revealed abundant stress-related motifs, including abscisic acid (ABA) and methyl jasmonate (MeJA) response elements, suggesting roles in abiotic stress adaptation. Transcriptome and qRT-PCR data confirmed that <i>AsNHX1/3/7/9/14/23/24/25/32</i> were up-regulated under salt stress, highlighting their importance in salinity tolerance.</p> Conclusions <p>This study provides the first comprehensive characterization of the <i>AsNHX</i> gene family, detailing their evolutionary, structural, and functional features. The findings offer critical insights into salt adaptation mechanisms in oat and identify promising targets for genetic improvement of salinity tolerance.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Genome-wide identification and expression profiling of the NHX gene family in oat (Avena sativa L.)

  • 期刊论文

  • 出版模式：

    开放获取

  • 发表日期：

    2026年1月29日

查看全文
  • Yuqi Li,
  • Meifeng Liu,
  • Wenjie Zhao,
  • Feng Yuan,
  • Zhenyu Jia,
  • Yi Wang,
  • Wendong Zheng,
  • Chengchen Pan,
  • Chunxiang Fu

摘要

Background

Salt stress imposes osmotic imbalance and ion toxicity, severely impairing crop growth and development. Na⁺/H⁺ antiporters (NHXs) play a central role in salt tolerance by mediating sodium transport across cellular membranes.

Results

We conducted a genome-wide characterization of the NHX gene family in four hexaploid oat cultivars, identifying 126 NHX genes. These were grouped into 33 orthologous gene groups (OGGs), comprising 10 core and 23 dispensable OGGs. The naked oat cultivar ‘Sanfensan (SFS)’ contained the largest number of both total and core NHX genes. Phylogenetic analysis classified AsNHX genes into three classes: vacuolar (Vac-class), endosomal (Endo-class), and plasma membrane (PM-class). Ka/Ks analysis indicated strong purifying selection across most members. Cis-regulatory element analysis revealed abundant stress-related motifs, including abscisic acid (ABA) and methyl jasmonate (MeJA) response elements, suggesting roles in abiotic stress adaptation. Transcriptome and qRT-PCR data confirmed that AsNHX1/3/7/9/14/23/24/25/32 were up-regulated under salt stress, highlighting their importance in salinity tolerance.

Conclusions

This study provides the first comprehensive characterization of the AsNHX gene family, detailing their evolutionary, structural, and functional features. The findings offer critical insights into salt adaptation mechanisms in oat and identify promising targets for genetic improvement of salinity tolerance.