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Background <p>The <i>MBD</i> gene family participates in key biological processes such as plant growth and development, hormonal signaling transduction, and stress response through epigenetic regulatory mechanisms. Broomcorn millet, an important drought-tolerant, barren-tolerant, and photoperiod-sensitive miscellaneous grain in northern China, the characteristics and functions of its <i>MBD</i> genes remain unclear.</p> Results <p>In this study, 11 members of the <i>MBD</i> gene family were identified. Collinearity analysis revealed that the <i>PmMBD</i> gene family primarily expanded through fragment duplication, with some members showing collinearity to genes in rice, wheat, and maize. The promoter regions of <i>PmMBDs</i> contain various cis-regulatory elements associated with developmental regulation, hormone responses, and stress responses, which may be closely related to their diverse regulatory functions. Transcriptome data and qRT-PCR analysis revealed that <i>PmMBD1</i>, <i>PmMBD2</i>, <i>PmMBD6</i>, and <i>PmMBD8</i> showed high expression levels in roots and leaves during the heading and jointing stages. qRT-PCR analysis under abiotic stress conditions revealed that <i>PmMBDs</i> exhibited significantly elevated expression in roots following ABA, salt, and PEG treatments.</p> Conclusions <p>The results of this study will help to further study the function of <i>PmMBDs</i> and provide important candidate gene resources and theoretical basis for the subsequent improvement of stress resistance and yield traits of broomcorn millet by molecular breeding methods.</p>

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Genome-wide identification and expression analysis of the MBD gene family in Broomcorn millet (Panicum miliaceum) and its response to abiotic stress

  • 期刊论文

  • 出版模式：

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  • 发表日期：

    2025年11月4日

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  • Yuanmeng Xu,
  • Jinrong Liu,
  • Huibin Qin,
  • Sichen Liu,
  • Ruiyun Wang,
  • Xiaoning Cao

摘要

Background

The MBD gene family participates in key biological processes such as plant growth and development, hormonal signaling transduction, and stress response through epigenetic regulatory mechanisms. Broomcorn millet, an important drought-tolerant, barren-tolerant, and photoperiod-sensitive miscellaneous grain in northern China, the characteristics and functions of its MBD genes remain unclear.

Results

In this study, 11 members of the MBD gene family were identified. Collinearity analysis revealed that the PmMBD gene family primarily expanded through fragment duplication, with some members showing collinearity to genes in rice, wheat, and maize. The promoter regions of PmMBDs contain various cis-regulatory elements associated with developmental regulation, hormone responses, and stress responses, which may be closely related to their diverse regulatory functions. Transcriptome data and qRT-PCR analysis revealed that PmMBD1, PmMBD2, PmMBD6, and PmMBD8 showed high expression levels in roots and leaves during the heading and jointing stages. qRT-PCR analysis under abiotic stress conditions revealed that PmMBDs exhibited significantly elevated expression in roots following ABA, salt, and PEG treatments.

Conclusions

The results of this study will help to further study the function of PmMBDs and provide important candidate gene resources and theoretical basis for the subsequent improvement of stress resistance and yield traits of broomcorn millet by molecular breeding methods.