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Background <p>Bananas are a globally significant crop, but their production is threatened by banana blood disease (BBD), caused by <i>Ralstonia syzygii</i> subsp. <i>celebesensis</i> (<i>Rsc</i>). While resistant cultivars like ‘Khai Pra Ta Bong’ (AAA genome) have been identified, the genetic basis of resistance remains unclear. This study uses RNA sequencing (RNA-seq) to investigate gene expression changes in ‘Khai Pra Ta Bong’ over seven days post-infection with <i>Rsc</i> isolate MY4101. Candidate genes associated with resistance were also evaluated in other cultivars, including resistant, moderately resistant, and highly susceptible cultivars.</p> Results <p>RNA-seq analysis identified several genes associated with BBD resistance mechanisms, showing significant upregulation as early as 12&#xa0;h post-inoculation. Key molecular processes, including xyloglucan endotransglucosylase hydrolases, receptor-like kinases, and glycine-rich proteins, were enriched at 24&#xa0;h post-inoculation, highlighting the activation of effector-triggered immunity (ETI). Quantitative real-time RT-PCR (qRT-PCR) validation further confirmed the differential expression of several key defense-related genes in other banana cultivars.</p> Conclusions <p>The study highlights the potential of these differentially expressed genes as genetic markers for BBD resistance, which could be valuable for breeding programs aimed at enhancing resistance in banana cultivars. These findings provide important insights into the molecular mechanisms of disease resistance and contribute to developing more efficient strategies for sustainable banana production and global food security.</p>

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Transcriptome analysis reveals key defense genes associated with resistance to banana blood disease

  • 期刊论文

  • 出版模式：

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  • 发表日期：

    2025年9月26日

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  • Thanwanit Thanyasiriwat,
  • Aphidech Sangdee,
  • Ladawan Rattanapolsan,
  • Praphat Kawicha,
  • Araya Arjcharoen Theanhom,
  • Ratri Boonruangrod,
  • Pumipat Tongyoo

摘要

Background

Bananas are a globally significant crop, but their production is threatened by banana blood disease (BBD), caused by Ralstonia syzygii subsp. celebesensis (Rsc). While resistant cultivars like ‘Khai Pra Ta Bong’ (AAA genome) have been identified, the genetic basis of resistance remains unclear. This study uses RNA sequencing (RNA-seq) to investigate gene expression changes in ‘Khai Pra Ta Bong’ over seven days post-infection with Rsc isolate MY4101. Candidate genes associated with resistance were also evaluated in other cultivars, including resistant, moderately resistant, and highly susceptible cultivars.

Results

RNA-seq analysis identified several genes associated with BBD resistance mechanisms, showing significant upregulation as early as 12 h post-inoculation. Key molecular processes, including xyloglucan endotransglucosylase hydrolases, receptor-like kinases, and glycine-rich proteins, were enriched at 24 h post-inoculation, highlighting the activation of effector-triggered immunity (ETI). Quantitative real-time RT-PCR (qRT-PCR) validation further confirmed the differential expression of several key defense-related genes in other banana cultivars.

Conclusions

The study highlights the potential of these differentially expressed genes as genetic markers for BBD resistance, which could be valuable for breeding programs aimed at enhancing resistance in banana cultivars. These findings provide important insights into the molecular mechanisms of disease resistance and contribute to developing more efficient strategies for sustainable banana production and global food security.