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Background <p>Skeletal muscle serves as the principal source of high-quality protein in human nutrition. Recent studies indicate that circRNAs play key roles in myogenesis by acting as competing endogenous RNAs (ceRNAs). However, the specific roles of circRNAs in myogenesis are largely unknown. Here, we attempted to systematically explore the functional role of circHOMER1 in myogenesis.</p> Results <p>The present study first demonstrated that circHOMER1 promotes the proliferation and differentiation of porcine skeletal muscle satellite cells (SMSCs). CircHOMER1 ceRNA regulatory network was constructed based on miRNA and mRNA sequencing. With immunofluorescence, dual-luciferase reporter gene, RIP, and rescue experiments, circHOMER1 was identified to promote porcine SMSCs differentiation by competitive binding miR-199b-5p to further inhibit <i>MAP3K11</i> expression. Moreover, in vivo experiments revealed that circHOMER1 accelerates muscle regeneration and development.</p> Conclusions <p>Collectively, circHOMER1 promotes the differentiation of SMSCs, muscle regeneration, and muscle development. In addition, a novel circHOMER1/miR-199b-5p/MAP3K11 regulatory network in porcine myogenesis was constructed. Our results provided a basis for in-depth analysis of the role of circRNAs in myogenesis.</p>

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CircHOMER1 in mediating porcine skeletal muscle development

  • 期刊论文

  • 出版模式：

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  • 发表日期：

    2025年9月26日

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  • Meng Li,
  • Yuxuan Gong,
  • Shuangji Ma,
  • Jiayi Chen,
  • Wanfeng Zhang,
  • Yang Yang,
  • Guoqing Cao,
  • Xiaohong Guo,
  • Bugao Li

摘要

Background

Skeletal muscle serves as the principal source of high-quality protein in human nutrition. Recent studies indicate that circRNAs play key roles in myogenesis by acting as competing endogenous RNAs (ceRNAs). However, the specific roles of circRNAs in myogenesis are largely unknown. Here, we attempted to systematically explore the functional role of circHOMER1 in myogenesis.

Results

The present study first demonstrated that circHOMER1 promotes the proliferation and differentiation of porcine skeletal muscle satellite cells (SMSCs). CircHOMER1 ceRNA regulatory network was constructed based on miRNA and mRNA sequencing. With immunofluorescence, dual-luciferase reporter gene, RIP, and rescue experiments, circHOMER1 was identified to promote porcine SMSCs differentiation by competitive binding miR-199b-5p to further inhibit MAP3K11 expression. Moreover, in vivo experiments revealed that circHOMER1 accelerates muscle regeneration and development.

Conclusions

Collectively, circHOMER1 promotes the differentiation of SMSCs, muscle regeneration, and muscle development. In addition, a novel circHOMER1/miR-199b-5p/MAP3K11 regulatory network in porcine myogenesis was constructed. Our results provided a basis for in-depth analysis of the role of circRNAs in myogenesis.