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Background <p>Intramuscular fat content is a key factor influencing pork quality. Previous studies by our group found that intramuscular injection of Nano VA into newborn piglets significantly increased Intramuscular fat content and improved pork quality. However, the regulatory mechanisms of noncoding RNAs in Intramuscular fat deposition remain unclear.</p> Results <p>To elucidate the mechanisms by which Nano VA affects pork quality via noncoding RNAs, whole transcriptome sequencing was performed on the longissimus dorsi muscle from control and Nano VA treated groups. A total of 90 differentially expressed circRNAs (DECs), 805 differentially expressed lncRNAs (DELs), and 58 differentially expressed miRNAs (DEmiRs) were screened between the two groups. Among these, 53 DECs were upregulated and 38 were downregulated; 430 DELs were upregulated and 375 were downregulated; 33 DEmiRs were upregulated and 25 were downregulated. GO and KEGG enrichment analyses indicated that the DECs, DELs, and DEmiRs were significantly enriched in pathways including insulin signaling, mTOR signaling, cGMP-PKG signaling, FoxO signaling, retinol metabolism, MAPK signaling, and autophagy. Based on the competitive endogenous RNA regulatory mechanism, a circRNA/lncRNA-miRNA-mRNA network associated with Intramuscular fat deposition was constructed, comprising 9 mRNAs, 3 miRNAs, 50 lncRNAs, and 9 circRNAs, including circRNA2640/circRNA11556-ssc-miR-1296-5p-<i>PIK</i>3<i>AP</i>1, MSTRG.543.4/MSTRG.1281.3 -ssc-miR-17-3p-XIAP, MSTRG.7503.15/circRNA11514-ssc-miR-17-3p-<i>ALDH</i>1<i>A</i>1 and MSTRG.12101.2/ciRNA113-ssc-miR-885-3-<i>GPAT</i>4. These findings suggest that Nano VA likely regulates Intramuscular fat deposition through the above ceRNA network.</p> Conclusions <p>This study reveals that Nano VA promotes porcine Intramuscular fat deposition by modulating an ncRNA mediated ceRNA network, providing a theoretical basis for understanding the molecular mechanisms by which Nano VA improves pork quality.</p>

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Molecular mechanism of nano-vitamin A-mediated regulation of intramuscular fat deposition involving noncoding RNAs in pigs

  • 期刊论文

  • 出版模式：

    开放获取

  • 发表日期：

    2025年8月2日

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  • Meng Li,
  • Wenrun Cai,
  • Ruotong Li,
  • Wenye Zhao,
  • Jiaxin Zhang,
  • Xiaoyin Rong,
  • Ting Zhang,
  • Yang Yang,
  • Bugao Li,
  • Xiaohong Guo

摘要

Background

Intramuscular fat content is a key factor influencing pork quality. Previous studies by our group found that intramuscular injection of Nano VA into newborn piglets significantly increased Intramuscular fat content and improved pork quality. However, the regulatory mechanisms of noncoding RNAs in Intramuscular fat deposition remain unclear.

Results

To elucidate the mechanisms by which Nano VA affects pork quality via noncoding RNAs, whole transcriptome sequencing was performed on the longissimus dorsi muscle from control and Nano VA treated groups. A total of 90 differentially expressed circRNAs (DECs), 805 differentially expressed lncRNAs (DELs), and 58 differentially expressed miRNAs (DEmiRs) were screened between the two groups. Among these, 53 DECs were upregulated and 38 were downregulated; 430 DELs were upregulated and 375 were downregulated; 33 DEmiRs were upregulated and 25 were downregulated. GO and KEGG enrichment analyses indicated that the DECs, DELs, and DEmiRs were significantly enriched in pathways including insulin signaling, mTOR signaling, cGMP-PKG signaling, FoxO signaling, retinol metabolism, MAPK signaling, and autophagy. Based on the competitive endogenous RNA regulatory mechanism, a circRNA/lncRNA-miRNA-mRNA network associated with Intramuscular fat deposition was constructed, comprising 9 mRNAs, 3 miRNAs, 50 lncRNAs, and 9 circRNAs, including circRNA2640/circRNA11556-ssc-miR-1296-5p-PIK3AP1, MSTRG.543.4/MSTRG.1281.3 -ssc-miR-17-3p-XIAP, MSTRG.7503.15/circRNA11514-ssc-miR-17-3p-ALDH1A1 and MSTRG.12101.2/ciRNA113-ssc-miR-885-3-GPAT4. These findings suggest that Nano VA likely regulates Intramuscular fat deposition through the above ceRNA network.

Conclusions

This study reveals that Nano VA promotes porcine Intramuscular fat deposition by modulating an ncRNA mediated ceRNA network, providing a theoretical basis for understanding the molecular mechanisms by which Nano VA improves pork quality.