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<p> <!--Query ID="Q1" Text="As keywords is required for this Journal. Please provide 3–6 keywords." Resolved="yes"--> <!--Query ID="Q2" Text="Please check and confirm Table 1 caption and footnote are correctly identify" Resolved="yes"--><i>Chrysochromulina parva</i> (<i>C. parva</i>) is a eukaryotic freshwater haptophyte algae found in lakes and rivers worldwide. It is known to be infected by viruses, yet knowledge of the diversity and activity of these viruses is still very limited. Based on sequences of PCR-amplified DNA polymerase B (<i>polB)</i> gene fragments, <i>Chrysochromulina parva</i> virus BQ1 (CpV-BQ1) was the first known lytic agent of <i>C. parva</i>, and was considered a member of the virus family <i>Phycodnaviridae</i>, order <i>Algavirales</i>. However, the genome of a different <i>C. parva</i>-infecting virus (CpV-BQ2, or <i>Tethysvirus ontarioense</i>) from another virus family, the <i>Mesomimiviridae</i>, order <i>Imitervirales</i>, was the first sequenced. Here, we report the complete genome sequence of the putative phycodnavirus CpV-BQ1, accession PQ783904. The complete CpV-BQ1 genome sequence is 165,454&#xa0;bp with a GC content of 32.32% and it encodes 193 open reading frames. Phylogenetic analyses of several virus hallmark genes including the <i>polB</i>, the late gene transcription factor (VLTF-3), and the putative A32-like virion packaging ATPase (Viral A32) all demonstrate that CpV-BQ1 is most closely related to other viruses in the phylum <i>Megaviricetes</i> within the order <i>Algavirales</i>, family <i>Phycodnaviridae</i>.</p>

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Hybrid sequencing reveals the genome of a Chrysochromulina parva virus and highlight its distinct replication strategy

  • 期刊论文

  • 出版模式：

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  • 发表日期：

    2025年5月17日

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  • Delaney Nash,
  • Christine N. Palermo,
  • Ichiro Inamoto,
  • Trevor C. Charles,
  • Jozef I. Nissimov,
  • Steven M. Short

摘要

Chrysochromulina parva (C. parva) is a eukaryotic freshwater haptophyte algae found in lakes and rivers worldwide. It is known to be infected by viruses, yet knowledge of the diversity and activity of these viruses is still very limited. Based on sequences of PCR-amplified DNA polymerase B (polB) gene fragments, Chrysochromulina parva virus BQ1 (CpV-BQ1) was the first known lytic agent of C. parva, and was considered a member of the virus family Phycodnaviridae, order Algavirales. However, the genome of a different C. parva-infecting virus (CpV-BQ2, or Tethysvirus ontarioense) from another virus family, the Mesomimiviridae, order Imitervirales, was the first sequenced. Here, we report the complete genome sequence of the putative phycodnavirus CpV-BQ1, accession PQ783904. The complete CpV-BQ1 genome sequence is 165,454 bp with a GC content of 32.32% and it encodes 193 open reading frames. Phylogenetic analyses of several virus hallmark genes including the polB, the late gene transcription factor (VLTF-3), and the putative A32-like virion packaging ATPase (Viral A32) all demonstrate that CpV-BQ1 is most closely related to other viruses in the phylum Megaviricetes within the order Algavirales, family Phycodnaviridae.