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Background <p>β-Glycosidases (BGLUs), belonging to the Glycoside Hydrolase Family 1 (GH1), play crucial roles in plant growth, development, and stress adaptation. While <i>BGLUs</i> have been extensively characterized in various plants, a comprehensive analysis of the <i>BGLU</i> family in <i>Brassica napus</i> has yet to be conducted.</p> Results <p>In this study, we identified 131 <i>BnaBGLU</i> genes in <i>B. napus</i> and classified them into 11 subfamilies. Gene duplication analysis revealed that the expansion of the <i>BnaBGLU</i> family primarily resulted from segmental duplications. Structural analysis revealed high conservation within subfamilies, while promoter cis-element profiling and transcriptional studies under phytohormone treatments and abiotic stresses demonstrated <i>BnaBGLUs</i> respond to diverse environmental stimuli. Additionally, <i>BnaBGLUs</i> exhibit tissue-specific expression patterns. Notably, <i>BnaBGLU27.C08</i> was significantly upregulated 96&#xa0;h post-inoculation with <i>Plasmodiophora brassicae</i> in the root of clubroot-resistant (CR) <i>B. napus</i> varieties. Furthermore, overexpression of <i>BnaBGLU27.C08</i> in Arabidopsis significantly enhanced resistance to clubroot disease.</p> Conclusions <p>These findings establish a foundation for further investigation on the <i>BnaBGLU</i> family in <i>B. napus</i> and offer potential candidate genes for breeding CR <i>B. napus</i> varieties.</p>

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Comprehensive analysis of Brassica napus Glycosyl hydrolase family 1 genes and functional analysis of BnaBGLU27.C08 in clubroot resistance

  • 期刊论文

  • 出版模式：

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  • 发表日期：

    2025年5月21日

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  • Yiji Shi,
  • Xiang Liu,
  • Shunjun Bao,
  • Ye Li,
  • Yuqing Shi,
  • Chen Liang,
  • Hongyu Sha,
  • Zihao Yu,
  • Qing Su,
  • Xiyu Zhang,
  • Xiaoyan Li,
  • Weinan Sun,
  • Chengyu Yu,
  • Keqi Li,
  • Zhen Huang

摘要

Background

β-Glycosidases (BGLUs), belonging to the Glycoside Hydrolase Family 1 (GH1), play crucial roles in plant growth, development, and stress adaptation. While BGLUs have been extensively characterized in various plants, a comprehensive analysis of the BGLU family in Brassica napus has yet to be conducted.

Results

In this study, we identified 131 BnaBGLU genes in B. napus and classified them into 11 subfamilies. Gene duplication analysis revealed that the expansion of the BnaBGLU family primarily resulted from segmental duplications. Structural analysis revealed high conservation within subfamilies, while promoter cis-element profiling and transcriptional studies under phytohormone treatments and abiotic stresses demonstrated BnaBGLUs respond to diverse environmental stimuli. Additionally, BnaBGLUs exhibit tissue-specific expression patterns. Notably, BnaBGLU27.C08 was significantly upregulated 96 h post-inoculation with Plasmodiophora brassicae in the root of clubroot-resistant (CR) B. napus varieties. Furthermore, overexpression of BnaBGLU27.C08 in Arabidopsis significantly enhanced resistance to clubroot disease.

Conclusions

These findings establish a foundation for further investigation on the BnaBGLU family in B. napus and offer potential candidate genes for breeding CR B. napus varieties.