Scholarlink

Background <p><i>Bacillus</i> is used as a biological control agent in agricultural production. The main mechanisms responsible for its biocontrol activity encompass the generation of various antifungal active substances during life activities, competition, antagonism with pathogens, promotion of growth, and induction of plant resistance, enhancing the inhibition of pathogenic fungi. <i>Bacillus</i> has high biological control potential and has become a research hotspot.</p> Results <p>It was found that strain KC14-1 had significant inhibitory effects on <i>Fusarium fujikuroi</i>, <i>Rhizoclonia solani</i>, <i>Alternaria solani</i>, <i>Fusarium oxysporum</i>, and <i>Valsa mali</i>. Based on morphological observations, physiological and biochemical determinations, and 16&#xa0;S rRNA, <i>gyrA</i>, and <i>gyrB</i> gene sequencing, strain KC14-1 was identified as <i>Bacillus subtilis</i>. Whole genome sequencing results showed that the genome of strain KC14-1 was composed of a ring chromosome 3,908,079&#xa0;bp in size, with a GC content of 43.82% and 3,895 coding genes. Anti-SMASH predicted that the genome of strain KC14-1 contained nine gene clusters that synthesised antibacterial substances. The homology between fengycin, bacillibactin, pulcherriminic acid, subtilosin A, and bacilysin was 100%.</p> Conclusion <p>The biocontrol potential of <i>Bacillus subtilis</i> KC14-1 was determined through whole-genome analysis. Our study provides a solid foundation for developing and utilising this strain.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Whole genome-sequence analysis of Bacillus subtilis strain KC14-1 with broad-spectrum antifungal activity

  • 期刊论文

  • 出版模式：

    开放获取

  • 发表日期：

    2025年3月31日

查看全文
  • Xiaowei Li,
  • Yahan Chen,
  • Shunyi Yang,
  • Yi Zhou,
  • Chengde Yang

摘要

Background

Bacillus is used as a biological control agent in agricultural production. The main mechanisms responsible for its biocontrol activity encompass the generation of various antifungal active substances during life activities, competition, antagonism with pathogens, promotion of growth, and induction of plant resistance, enhancing the inhibition of pathogenic fungi. Bacillus has high biological control potential and has become a research hotspot.

Results

It was found that strain KC14-1 had significant inhibitory effects on Fusarium fujikuroi, Rhizoclonia solani, Alternaria solani, Fusarium oxysporum, and Valsa mali. Based on morphological observations, physiological and biochemical determinations, and 16 S rRNA, gyrA, and gyrB gene sequencing, strain KC14-1 was identified as Bacillus subtilis. Whole genome sequencing results showed that the genome of strain KC14-1 was composed of a ring chromosome 3,908,079 bp in size, with a GC content of 43.82% and 3,895 coding genes. Anti-SMASH predicted that the genome of strain KC14-1 contained nine gene clusters that synthesised antibacterial substances. The homology between fengycin, bacillibactin, pulcherriminic acid, subtilosin A, and bacilysin was 100%.

Conclusion

The biocontrol potential of Bacillus subtilis KC14-1 was determined through whole-genome analysis. Our study provides a solid foundation for developing and utilising this strain.