Genome-wide identification of R2R3-MYB transcription factor subfamily genes involved in salt stress in rice (Oryza sativa L.)

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2024年8月23日

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Hao-Cheng Zhang,
Yuan-Hang Gong,
Tao Tao,
Shuai Lu,
Wen-Yu Zhou,
Han Xia,
Xin-Yi Zhang,
Qing-Qing Yang,
Ming-Qiu Zhang,
Lian-Min Hong,
Qian-Qian Guo,
Xin-Zhe Ren,
Zhi-Di Yang,
Xiu-Ling Cai,
De-Yong Ren,
Ji-Ping Gao,
Su-Kui Jin,
Yu-Jia Leng

摘要

Background

R2R3-MYB transcription factors belong to one of the largest gene subfamilies in plants, and they are involved in diverse biological processes. However, the role of R2R3-MYB transcription factor subfamily genes in the response of rice (Oryza sativa L.) to salt stress has been rarely reported.

Results

In this study, we performed a genome-wide characterization and expression identification of rice R2R3-MYB transcription factor subfamily genes. We identified a total of 117 R2R3-MYB genes in rice and characterized their gene structure, chromosomal location, and cis-regulatory elements. According to the phylogenetic relationships and amino acid sequence homologies, the R2R3-MYB genes were divided into four groups. qRT-PCR of the R2R3-MYB genes showed that the expression levels of 10 genes significantly increased after 3 days of 0.8% NaCl treatment. We selected a high expression gene OsMYB2-115 for further analysis. OsMYB2-115 was highly expressed in the roots, stem, leaf, and leaf sheath. OsMYB2-115 was found to be localized in the nucleus, and the yeast hybrid assay showed that OsMYB2-115 has transcriptional activation activity.

Conclusion

This result provides important information for the functional analyses of rice R2R3-MYB transcription factor subfamily genes related to the salt stress response and reveals that OsMYB2-115 may be an important gene associated with salt tolerance in rice.