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Objectives <p><i>Haliotis discus hannai</i>, a primary aquaculture species in East Asia, is highly susceptible to viral infections, such as abalone herpesvirus. To facilitate the understanding of antiviral immune mechanisms, we generated a hemocyte transcriptome dataset following stimulation with Polyinosinic: polycytidylic acid (poly[I: C]), a synthetic analog of viral double-stranded RNA. This dataset offers a molecular basis for assessing immune-related genes and pathways in abalone.</p> Data description <p>Adult abalones were injected with poly(I:C), and hemocytes were collected at 2 and 7 days post-infection, along with uninfected controls. Total RNA was extracted and sequenced using the Illumina paired-end platform. After quality filtering, <i>de novo</i> assembly with Trinity produced 281,050 transcripts and 214,676 unigenes (N50: 722&#xa0;bp and GC content: 41.06%). Open reading frames predicted by TransDecoder yielded 18,371 coding sequences. Functional annotation against Non-Redundant Protein, Universal Protein Resource, Protein Families, Gene Ontology, and Kyoto Encyclopedia of Genes and Genomes databases identified numerous immune-related genes The raw reads, assembled transcriptome, and annotation files are publicly available in the National Center for Biotechnology Information Sequence Read Archive and Figshare, providing a valuable reference for future studies on the innate immunity of <i>H. discus hannai.</i></p>

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De novo transcriptome assembly of hemocytes from Haliotis discus hannai following poly(I:C) stimulation

  • 期刊论文

  • 出版模式：

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  • 发表日期：

    2026年1月9日

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  • Seung Hyun Yoo,
  • Jeong Gyu Kim,
  • Ha Eun Lee,
  • Yong Jun Park,
  • Yong Bae Seo,
  • Mi Jeong Jo,
  • Jong-Oh Kim,
  • Gun-Do Kim

摘要

Objectives

Haliotis discus hannai, a primary aquaculture species in East Asia, is highly susceptible to viral infections, such as abalone herpesvirus. To facilitate the understanding of antiviral immune mechanisms, we generated a hemocyte transcriptome dataset following stimulation with Polyinosinic: polycytidylic acid (poly[I: C]), a synthetic analog of viral double-stranded RNA. This dataset offers a molecular basis for assessing immune-related genes and pathways in abalone.

Data description

Adult abalones were injected with poly(I:C), and hemocytes were collected at 2 and 7 days post-infection, along with uninfected controls. Total RNA was extracted and sequenced using the Illumina paired-end platform. After quality filtering, de novo assembly with Trinity produced 281,050 transcripts and 214,676 unigenes (N50: 722 bp and GC content: 41.06%). Open reading frames predicted by TransDecoder yielded 18,371 coding sequences. Functional annotation against Non-Redundant Protein, Universal Protein Resource, Protein Families, Gene Ontology, and Kyoto Encyclopedia of Genes and Genomes databases identified numerous immune-related genes The raw reads, assembled transcriptome, and annotation files are publicly available in the National Center for Biotechnology Information Sequence Read Archive and Figshare, providing a valuable reference for future studies on the innate immunity of H. discus hannai.