Scholarlink

Background <p>Adherent-invasive <i>Escherichia coli</i> (AIEC) is linked to intestinal inflammation in inflammatory bowel disease (IBD). <i>Arthrospira platensis</i> and <i>Lactobacillus helveticus</i> exhibit anti-inflammatory properties individually, yet their effects remain underexplored in IBD-associated inflammation. We aimed to investigate the anti-inflammatory potential of <i>L. helveticus</i> and the hydroalcoholic extract of <i>A. platensis</i> (HA-<i>A. platensis</i>) in Caco-2 cells inflamed by IBD-associated <i>E. coli</i>.</p> Methods <p>Caco-2 cells inflamed by a Crohn’s disease (CD)-associated <i>E. coli</i> strain (MOI 10) were treated with HA-A. platensis (2&#xa0;mg/mL) and/or <i>L. helveticus</i> (MOI 50) in live (LBC), heat-killed (HKC), or cell-free supernatant (CFS) forms. The anti-invasion/adhesion properties of <i>L. helveticus</i> and/or HA-<i>A. platensis</i> were investigated by assessing the CD-associated <i>E. coli</i> invasion/adhesion rate (%). Signaling molecules (NF-κB, STAT3, NOD2) were analyzed via qPCR to capture pathway activation dynamics, while cytokines (TNF-α, IL-1β, IL-8, IL-10) were quantified by ELISA to assess secreted functional proteins.</p> Results <p>HA-<i>A. platensis</i> reduced <i>E. coli</i> adhesion by 68% (<i>P</i> &lt; 0.001) and completely inhibited invasion. <i>L. helveticus</i> (live form) decreased adhesion by 88% and invasion by 90%. Combined treatment showed synergistic effects, reducing adhesion by 89% and fully blocking invasion. HA-<i>A. platensis</i> downregulated STAT3 expression by 0.4-fold (<i>P</i> &lt; 0.01), while <i>L. helveticus</i> (heat-killed form) reduced NF-κB by 0.51-fold (<i>P</i> &lt; 0.05) and increased NOD2 by 1.8-fold (<i>P</i> &lt; 0.01). Cytokine analysis revealed that HA-<i>A. platensis</i> decreased IL-1β by 0.61-fold (<i>P</i> &lt; 0.001), and <i>L. helveticus</i> (heat-killed) reduced TNF-α (0.51-fold) and IL-8 (0.23-fold) while elevating anti-inflammatory IL-10 (4.39-fold; <i>P</i> &lt; 0.001).</p> Conclusions <p><i>L. helveticus</i> and HA-<i>A. platensis</i> synergistically inhibit CD-associated <i>E. coli</i> pathogenicity and modulate inflammatory responses in vitro. These findings highlight their potential as adjunctive therapies for CD, warranting further preclinical validation.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Anti-Inflammatory effects of Lactobacillus helveticus and Arthrospira platensis on colonic cells inflamed by Crohn’s disease-associated Escherichia coli

  • 期刊论文

  • 出版模式：

    开放获取

  • 发表日期：

    2025年6月11日

查看全文
  • Samira Alipour,
  • Hossein Halimi,
  • Nastaran Asri,
  • Mohammad Rostami-Nejad,
  • Leila Pishkar,
  • Hamidreza Houri

摘要

Background

Adherent-invasive Escherichia coli (AIEC) is linked to intestinal inflammation in inflammatory bowel disease (IBD). Arthrospira platensis and Lactobacillus helveticus exhibit anti-inflammatory properties individually, yet their effects remain underexplored in IBD-associated inflammation. We aimed to investigate the anti-inflammatory potential of L. helveticus and the hydroalcoholic extract of A. platensis (HA-A. platensis) in Caco-2 cells inflamed by IBD-associated E. coli.

Methods

Caco-2 cells inflamed by a Crohn’s disease (CD)-associated E. coli strain (MOI 10) were treated with HA-A. platensis (2 mg/mL) and/or L. helveticus (MOI 50) in live (LBC), heat-killed (HKC), or cell-free supernatant (CFS) forms. The anti-invasion/adhesion properties of L. helveticus and/or HA-A. platensis were investigated by assessing the CD-associated E. coli invasion/adhesion rate (%). Signaling molecules (NF-κB, STAT3, NOD2) were analyzed via qPCR to capture pathway activation dynamics, while cytokines (TNF-α, IL-1β, IL-8, IL-10) were quantified by ELISA to assess secreted functional proteins.

Results

HA-A. platensis reduced E. coli adhesion by 68% (P < 0.001) and completely inhibited invasion. L. helveticus (live form) decreased adhesion by 88% and invasion by 90%. Combined treatment showed synergistic effects, reducing adhesion by 89% and fully blocking invasion. HA-A. platensis downregulated STAT3 expression by 0.4-fold (P < 0.01), while L. helveticus (heat-killed form) reduced NF-κB by 0.51-fold (P < 0.05) and increased NOD2 by 1.8-fold (P < 0.01). Cytokine analysis revealed that HA-A. platensis decreased IL-1β by 0.61-fold (P < 0.001), and L. helveticus (heat-killed) reduced TNF-α (0.51-fold) and IL-8 (0.23-fold) while elevating anti-inflammatory IL-10 (4.39-fold; P < 0.001).

Conclusions

L. helveticus and HA-A. platensis synergistically inhibit CD-associated E. coli pathogenicity and modulate inflammatory responses in vitro. These findings highlight their potential as adjunctive therapies for CD, warranting further preclinical validation.