Scholarlink

<p>Salt stress is a major environmental challenge for global rice production because it disrupts ionic balance and induces oxidative damage. We investigated the role of the rice BAHD-acyltransferase-like protein gene <i>OsDCR</i> in the salt stress response and found that <i>OsDCR</i>-overexpressing plants showed enhanced salt tolerance. In contrast, CRISPR/Cas9-mediated-knockout rice lines were sensitive to salt stress. <i>OsDCR</i>-OE plants also exhibited reduced oxidative stress and high ABA accumulation under 150 mM NaCl, and quantitative RT-PCR analysis revealed that the expression of <i>OsDCR</i> was induced by salt stress and high temperature. Additionally, we identified a proline-rich protein, OsPRP3, as an interaction partner of OsDCR by yeast two-hybrid, LCI and Co-IP assays. Similar to OsDCR, OsPRP3 also positively regulated rice salt tolerance and acted downstream of OsDCR. Taken together, our findings suggest that OsDCR functions as a positive regulator of salt tolerance by modulating ABA biosynthesis and interacting with OsPRP3. Our results regarding the molecular role of <i>OsDCR</i> provide novel insights for developing salt-tolerant rice cultivars.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Overexpression of the BAHD Acyltransferase-Like Protein gene OsDCR Enhances Salt Tolerance in Rice

  • 期刊论文

  • 出版模式：

    开放获取

  • 发表日期：

    2026年5月21日

查看全文
  • Xi Liu,
  • Linqian Li,
  • Mengjie Xu,
  • Nan Lu,
  • Yunlong Zhang,
  • Xinhe Wang,
  • Rui Shi,
  • Fang Wang,
  • Jianhui Ji,
  • Qingsong Gao,
  • Maodi Zhu,
  • Haibo Dai

摘要

Salt stress is a major environmental challenge for global rice production because it disrupts ionic balance and induces oxidative damage. We investigated the role of the rice BAHD-acyltransferase-like protein gene OsDCR in the salt stress response and found that OsDCR-overexpressing plants showed enhanced salt tolerance. In contrast, CRISPR/Cas9-mediated-knockout rice lines were sensitive to salt stress. OsDCR-OE plants also exhibited reduced oxidative stress and high ABA accumulation under 150 mM NaCl, and quantitative RT-PCR analysis revealed that the expression of OsDCR was induced by salt stress and high temperature. Additionally, we identified a proline-rich protein, OsPRP3, as an interaction partner of OsDCR by yeast two-hybrid, LCI and Co-IP assays. Similar to OsDCR, OsPRP3 also positively regulated rice salt tolerance and acted downstream of OsDCR. Taken together, our findings suggest that OsDCR functions as a positive regulator of salt tolerance by modulating ABA biosynthesis and interacting with OsPRP3. Our results regarding the molecular role of OsDCR provide novel insights for developing salt-tolerant rice cultivars.