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<p><i>Helicobacter pylori (H. pylori)</i> is a gram-negative microaerophilic bacterium that secretes several endotoxins to induce stomach and duodenum diseases. Herein, a molecularly imprinted polymer (MIP) based electrochemical and immuno-sensor was developed for the determination of <i>H. pylori-specific</i> BabA antigen (<sup>BabA</sup>Ag) and antibody (<sup>BabA</sup>Ab), respectively. The foremost step is the electropolymerization of palladium nanoparticles (PdNPs) and poly (3,4-ethylenedioxythiophene) (PEDOT) onto the electrode surface. The second step involves the fabrication of an electropolymerization-based <sup>BabA</sup>Ag recognition platform (MIP) at the screen-printed gold electrode surface. Thirdly, anti-<sup>BabA</sup>Ab was used to further cross-validate MIP<sub>BabA</sub> and <sup>BabA</sup>Ag binding on the fabricated electrode. The fabricated MIP<sub>BabA</sub>/PdNPs/PEDOT sensor detects the variable concentrations of <sup>BabA</sup>Ag under electrochemical studies. The imprinted polymer was characterized with scanning electron microscopy, Brunauer–Emmett–Teller analysis, and X-ray photoelectron spectroscopy techniques. The electrochemical studies demonstrated the high sensitivity of the sensor with a low detection limit of 0.1&#xa0;ng/ml in a linear dynamic range of 0.1–50&#xa0;ng/ml BabA. The prospect of work includes the integration of a developed detection technique with a lab-on-a-chip kit, as accurate and early detection of <i>H. pylori</i>-associated biomarkers such as BabA is important for timely diagnosis and monitoring of gastric disorders, including gastric cancer.</p>

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Advancing palladium PEDOT molecularly imprinted nanoelectrochemical sensing interface for selective dual detection of Helicobacter pylori BabA antigen and antibody

  • 期刊论文

  • 出版模式：

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  • 发表日期：

    2026年7月2日

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  • Nidhi Chauhan,
  • Kirti Saxena,
  • Smriti Gaba,
  • Shringika Soni,
  • Bansi Dhar Malhotra,
  • Utkarsh Jain

摘要

Helicobacter pylori (H. pylori) is a gram-negative microaerophilic bacterium that secretes several endotoxins to induce stomach and duodenum diseases. Herein, a molecularly imprinted polymer (MIP) based electrochemical and immuno-sensor was developed for the determination of H. pylori-specific BabA antigen (BabAAg) and antibody (BabAAb), respectively. The foremost step is the electropolymerization of palladium nanoparticles (PdNPs) and poly (3,4-ethylenedioxythiophene) (PEDOT) onto the electrode surface. The second step involves the fabrication of an electropolymerization-based BabAAg recognition platform (MIP) at the screen-printed gold electrode surface. Thirdly, anti-BabAAb was used to further cross-validate MIPBabA and BabAAg binding on the fabricated electrode. The fabricated MIPBabA/PdNPs/PEDOT sensor detects the variable concentrations of BabAAg under electrochemical studies. The imprinted polymer was characterized with scanning electron microscopy, Brunauer–Emmett–Teller analysis, and X-ray photoelectron spectroscopy techniques. The electrochemical studies demonstrated the high sensitivity of the sensor with a low detection limit of 0.1 ng/ml in a linear dynamic range of 0.1–50 ng/ml BabA. The prospect of work includes the integration of a developed detection technique with a lab-on-a-chip kit, as accurate and early detection of H. pylori-associated biomarkers such as BabA is important for timely diagnosis and monitoring of gastric disorders, including gastric cancer.