Exploring the bioactive potential of green synthesized silver nanoparticles coated serratiopeptidase and chalcone derivatives

Serratiopeptidase is a proteolytic enzyme that has gained considerable interest in the medical and pharmaceutical industries due to its analgesic, fibrinolytic, and anti-inflammatory effects. Silver nanoparticles (AgNPs) were coated on Serratiopeptidase to analyze and compare its bioactivity along with the bioactivity of newly synthesized chalcone derivatives.

In the present study, two novel chalcone derivatives were successfully synthesized and evaluated for their biological activities. Among the synthesized compounds, VIT-A3S exhibited the highest proteolytic activity (231.63 ± 0.43 U/mL) and anti-inflammatory activities (92.67 ± 0.48%), indicating its therapeutic potential. The structural elucidation of VIT-A3S was carried out using spectroscopic techniques, including Nuclear Magnetic Resonance (NMR), Fourier Transform Infrared Spectroscopy (FTIR), and Ultraviolet–Visible (UV–Vis) spectroscopy, confirming its molecular architecture. Furthermore, silver nanoparticles (Ag NPs) were synthesized and characterized, with their colloidal stability assessed using zeta potential analysis. The integrated approach combining synthetic chemistry, biological evaluation and nanotechnology highlighted the potential of VIT-A3S for further pharmaceutical development. Overall, the study highlighted that the chalcone derivatives (VIT-A3S and VIT-PAGS) possessed a broad spectrum of biological activities including antioxidant, anti-inflammatory, and protease functions, with significantly higher potency compared to serratiopeptidase based formulations.

These findings suggest that chalcone derivatives represent a promising class of therapeutic agents, with potential application in the management of inflammation-related disorders and warrant further in-depth pharmacological and clinical investigations.