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<p>Bacterial infections are common and challenging to treat due to increasing antibiotic resistance. Research has focused on using various nanoparticles, with studies showing that Selenium Nanoparticles (Se-NPs) have antibacterial effects. This study focuses on the biosynthesis and characterization of Se-NPs using <i>Pseudomonas aeruginosa</i> and <i>Bacillus pumilus</i> RMO6. Bacteria were cultivated on the microbial culture media, followed by the synthesis of Se-NPs through the reduction of sodium selenite in the bacterial supernatant. Characterization techniques, including UV-Vis spectroscopy, FT-IR, XRD, TEM imaging, and DLS, confirmed the successful formation of Se-NPs, revealing their size, morphology, and surface properties. The Biosynthesized nanoparticles exhibited significant antimicrobial activity against four standard bacterial strains: <i>Escherichia coli</i>,<i> Pseudomonas aeruginosa</i>,<i> Staphylococcus aureus</i>, and <i>Enterococcus faecalis</i>. Notably, Se-NPs synthesized by <i>Bacillus pumilus</i> demonstrated a significantly lower MIC and greater effectiveness in biofilm eradication (MBEC) compared to those produced by <i>Pseudomonas aeruginosa</i>, with a fourfold reduction in MIC against <i>Escherichia coli</i> and <i>Staphylococcus aureus</i>. These findings highlight the potential of Se-NPs as effective agents in combating resistant bacterial infections and suggest their promising application in medical treatments.</p>

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Biosynthesis and antibacterial activity of selenium nanoparticles using Pseudomonas aeruginosa and Bacillus pumilus RMO6

  • 期刊论文

  • 出版模式：

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  • 发表日期：

    2026年4月9日

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  • Elina Heidari,
  • Morteza Milani,
  • Farhad Bani,
  • Faezeh Milani,
  • Abolfazl Akbarzadeh

摘要

Bacterial infections are common and challenging to treat due to increasing antibiotic resistance. Research has focused on using various nanoparticles, with studies showing that Selenium Nanoparticles (Se-NPs) have antibacterial effects. This study focuses on the biosynthesis and characterization of Se-NPs using Pseudomonas aeruginosa and Bacillus pumilus RMO6. Bacteria were cultivated on the microbial culture media, followed by the synthesis of Se-NPs through the reduction of sodium selenite in the bacterial supernatant. Characterization techniques, including UV-Vis spectroscopy, FT-IR, XRD, TEM imaging, and DLS, confirmed the successful formation of Se-NPs, revealing their size, morphology, and surface properties. The Biosynthesized nanoparticles exhibited significant antimicrobial activity against four standard bacterial strains: Escherichia coli, Pseudomonas aeruginosa, Staphylococcus aureus, and Enterococcus faecalis. Notably, Se-NPs synthesized by Bacillus pumilus demonstrated a significantly lower MIC and greater effectiveness in biofilm eradication (MBEC) compared to those produced by Pseudomonas aeruginosa, with a fourfold reduction in MIC against Escherichia coli and Staphylococcus aureus. These findings highlight the potential of Se-NPs as effective agents in combating resistant bacterial infections and suggest their promising application in medical treatments.