Scholarlink

Background <p>IgA-mediated vasculitis (IgAV) is a complex inflammatory disease. Unravelling its genetic background would allow us to identify genetic biomarkers that may be used as additional tools in its daily management, helping to solve the clinical challenge that this vasculitis entails. C5 is a potent immune mediator that is proteolytically processed to generate C5a, a potent anaphylatoxin that exerts its function via C5aR1. <i>C5</i> downstream variants (rs3761847 and rs10818488) have been recently related to IgAV pathogenesis. Additionally, C5a and C5aR1 dysregulation contributes to the development of inflammatory diseases, and, particularly, elevated C5a plasma levels have been observed in IgAV patients in the acute stage. Accordingly, we aimed to evaluate the influence of <i>C5</i> and <i>C5AR1</i> on the pathophysiology of IgAV.</p> Methods <p>Eight <i>C5</i> (rs10760128, rs74971050, rs4310279, rs7868761, rs10818495, rs10156396, rs3815467, and rs16910280) and three <i>C5AR1</i> (rs10853784, rs11673071, and rs11670789) tag variants were genotyped in 342 Caucasian IgAV patients and 723 ethnically matched healthy controls.</p> Results <p>No statistically significant differences were observed when <i>C5</i> and <i>C5AR1</i> frequencies were compared between IgAV patients and healthy controls. Likewise, similar <i>C5</i> and <i>C5AR1</i> frequencies were observed amongst IgAV patients stratified according to IgAV severity (presence/absence of nephritis). Furthermore, no <i>C5</i> and <i>C5AR1</i> differences were disclosed when IgAV patients were stratified according to demographic and clinical IgAV characteristics other than nephritis (age at disease onset, presence/absence of joint and gastrointestinal manifestations) and sex.</p> Conclusions <p>Our results suggest that <i>C5</i> and <i>C5AR1</i> are not related to IgAV pathogenesis and, therefore, these genes may not be useful as IgAV genetic biomarkers.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Evaluating the usefulness of C5 and C5AR1 as genetic biomarkers of IgA-mediated vasculitis

  • 期刊论文

  • 出版模式：

    开放获取

  • 发表日期：

    2025年7月27日

查看全文
  • Joao Carlos Batista-Liz,
  • Vanesa Calvo-Río,
  • María Sebastián Mora-Gil,
  • María Teresa Leonardo,
  • Ana Cristina Peñalba,
  • Luis Martín-Penagos,
  • Javier Narváez,
  • Belén Sevilla-Pérez,
  • José Luis Callejas-Rubio,
  • Ligia Gabrie,
  • Rafael Gálvez Sánchez,
  • Luis Caminal-Montero,
  • Paz Collado,
  • María José Rodríguez Valls,
  • Diego de Argila,
  • Patricia Quiroga-Colina,
  • Esther Francisca Vicente-Rabaneda,
  • Esteban Rubio,
  • Manuel León Luque,
  • Juan María Blanco-Madrigal,
  • Eva Galíndez-Agirregoikoa,
  • Ricardo Blanco,
  • Verónica Pulito-Cueto,
  • Raquel López-Mejías

摘要

Background

IgA-mediated vasculitis (IgAV) is a complex inflammatory disease. Unravelling its genetic background would allow us to identify genetic biomarkers that may be used as additional tools in its daily management, helping to solve the clinical challenge that this vasculitis entails. C5 is a potent immune mediator that is proteolytically processed to generate C5a, a potent anaphylatoxin that exerts its function via C5aR1. C5 downstream variants (rs3761847 and rs10818488) have been recently related to IgAV pathogenesis. Additionally, C5a and C5aR1 dysregulation contributes to the development of inflammatory diseases, and, particularly, elevated C5a plasma levels have been observed in IgAV patients in the acute stage. Accordingly, we aimed to evaluate the influence of C5 and C5AR1 on the pathophysiology of IgAV.

Methods

Eight C5 (rs10760128, rs74971050, rs4310279, rs7868761, rs10818495, rs10156396, rs3815467, and rs16910280) and three C5AR1 (rs10853784, rs11673071, and rs11670789) tag variants were genotyped in 342 Caucasian IgAV patients and 723 ethnically matched healthy controls.

Results

No statistically significant differences were observed when C5 and C5AR1 frequencies were compared between IgAV patients and healthy controls. Likewise, similar C5 and C5AR1 frequencies were observed amongst IgAV patients stratified according to IgAV severity (presence/absence of nephritis). Furthermore, no C5 and C5AR1 differences were disclosed when IgAV patients were stratified according to demographic and clinical IgAV characteristics other than nephritis (age at disease onset, presence/absence of joint and gastrointestinal manifestations) and sex.

Conclusions

Our results suggest that C5 and C5AR1 are not related to IgAV pathogenesis and, therefore, these genes may not be useful as IgAV genetic biomarkers.