Small noncoding RNAs and sperm nuclear basic proteins reflect the environmental impact on germ cells

期刊论文
出版模式：

开放获取
发表日期：

2024年1月20日

查看全文

Giulio Ferrero,
Rosaria Festa,
Laura Follia,
Gennaro Lettieri,
Sonia Tarallo,
Tiziana Notari,
Antonella Giarra,
Carmela Marinaro,
Barbara Pardini,
Alessandra Marano,
Giulia Piaggeschi,
Carla Di Battista,
Marco Trifuoggi,
Marina Piscopo,
Luigi Montano,
Alessio Naccarati

摘要

Background

Molecular techniques can complement conventional spermiogram analyses to provide new information on the fertilizing potential of spermatozoa and to identify early alterations due to environmental pollution.

Methods

Here, we present a multilevel molecular profiling by small RNA sequencing and sperm nuclear basic protein analysis of male germ cells from 33 healthy young subjects residing in low and high-polluted areas.

Results

Although sperm motility and sperm concentration were comparable between samples from the two sites, those from the high-pollution area had a higher concentration of immature/immune cells, a lower protamine/histone ratio, a reduced ability of sperm nuclear basic proteins to protect DNA from oxidative damage, and an altered copper/zinc ratio in sperm. Sperm levels of 32 microRNAs involved in intraflagellar transport, oxidative stress response, and spermatogenesis were different between the two areas. In parallel, a decrease of Piwi-interacting RNA levels was observed in samples from the high-polluted area.

Conclusions

This comprehensive analysis provides new insights into pollution-driven epigenetic alterations in sperm not detectable by spermiogram.

Graphical Abstract