Reference Genes for the Real-Time PCR Analysis of Relative Gene Expression in Various Human Myocardial Pathologies

Active study of the molecular mechanisms of the occurrence and development of various cardiac pathologies using the assessment of gene expression by real-time PCR is necessary to solve the problem of finding and selecting adequate reference genes. A search is conducted for the most stably expressed potential reference genes in the intravital myocardial samples of patients with severe heart failure due to hypertrophic cardiomyopathy or coronary heart disease. Gene-expression analysis is performed using reverse transcription and real-time PCR. Gene-expression stability is assessed using the RefFinder resource. It is shown that the genes expressed most stably in human myocardium in the case of heart failure are PSMD6, PSMD7, SARS1, and AARS1. In this regard, we recommend including them in the gene panel when searching for and validating potential reference genes to assess the expression of candidate genes in myocardial tissues.