Synthesis of Albumin Nanoparticles with Immobilized and Incorporated Fluorophores and Drugs, Properties and Release Profiles

The synthesis of albumin nanoparticles was performed sequentially using the methods of pH-coacervation, desolvation and cross-linking with glutaraldehyde. The resulting particles have a shape close to spherical. A dependence of the sizes of the resulting particles on the desolvation rate was described. Immobilization of two fluorescent dyes, indocyanine green and sodium fluorescein, as well as the anti-ischemic drug quinacrine, was carried out on the surface of nanoparticles, which made it possible to obtain prolonged release forms. For quinacrine immobilized on the surface of nanoparticles, noticeable release of quinacrine ends by 30 h from the start of the experiment and accounts for 65% of the immobilized active substance. Fluorescent dyes were not released during this period.