Cyto-Histological Differentiation of the Intestine at Different Stages of Development in Larvae of Ornamental Fish (Pterophyllum scalare)
摘要
The histological development of the digestive system in Pterophyllum scalare larvae was studied under controlled conditions (30 ± 2°C). Eggs were incubated, and samples from 0 to 25 days post-hatching (dph) were processed for analysis. Developmental stages (preflexion, flexion, postflexion) were determined based on notochord flexion. At hatching (0 dph), the gut was a straight tube dorsal to the yolk sac. By 7 dph, the bucco-pharynx showed squamous epithelium, oral valves, taste buds, and goblet cells. Gastric differentiation started at 2 dph, with the formation of Gastric glands by 11 dph, and gets completed by 24 dph. Initially a simple tube at 1 dph, the intestinal development of mucosal folds and formation of supranucleolar vacuoles by 7–9 dph was evident. These findings help refine larval-rearing protocols, address developmental bottlenecks, and improve weaning processes.