Changes in the Content of microRNA775A and Its Role in Post-Transcriptional Regulation of Targeted Genes in Corn Leaves under Hypoxia

Under the influence of hypoxia in plants, the transcription of genes responsible for adaptation to stress caused by low oxygen levels changes. Changes in metabolic pathways under stress conditions may be regulated by microRNAs. It was found that the content of microRNA775A increases in corn leaves under the influence of hypoxia. The use of the fluorescent probe miR775A-ROX made it possible to establish an increase in the number of RNA-inducing silencing complexes (RISC), formed on the basis of microRNA775A, in maize leaves during the development of hypoxic stress. The results we obtained indicate that microRNA775A is involved in the processes of adaptation of the body to hypoxic conditions by regulating the expression of target genes at the post-transcriptional level using the RNA interference mechanism.