Effect of the Radiation within Terahertz Range on the Transport Properties of Albumin: Binding with Metal Ions

The effect of terahertz radiation on clusterization of bovine serum albumin (BSA) molecules and on BSA binding with nickel, cobalt and cadmium ions is investigated by means of high performance liquid chromatography and EPR spectroscopy under variation of the concentration of molecular oxygen in solution. Irradiation is detected to remove steric hindrance for oxygen adsorption. The degree of nickel and cobalt ion binding with irradiated BSA samples is substantially higher than with non-irradiated ones, while for cadmium the binding degree is the same and rather low in both cases. The functional groups in BSA molecule participating in metal ion binding are revealed by means of semi-empirical simulation.