Saliva Glucose Determination by Biosensor: Method Comparison to Chemiluminescence Immunoassay

The noninvasive sampling materials and methods have garnered great interest among researchers. Saliva is considered the most popular material, and glucose is one of the most commonly used biochemical parameters. This article provides a comparison between the biosensor and autoanalyzer methods for saliva glucose measurement and identifies its potential as a point-of-care testing tool. The experiments were performed using salivary samples from twenty-six adults collected using saliva collection tubes. The swabs were soaked in saliva for 1 min and then centrifuged for 10 min at 1500 g. Glucose concentrations were determined using two methods: one based on an autoanalyzer using chemiluminescence assay and the other based on a biosensor using glucose oxidase enzyme-based amperometric method. A statistically significant correlation was found between the two methods (r = 0.802; p < 0.001). The most remarkable result from the data is that there were no significant differences in saliva glucose measurements between the two methods. This paper presents an innovative biosensor method that can be readily used in practice for rapid results, low cost, and specificity compared to the frequently used autoanalyzer method.