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Abstract <p>Functional unloading of skeletal muscles is observed during space flight, prolonged bed rest, or limb immobilization. This leads to skeletal muscle atrophy, which has serious health consequences and significantly reduces quality of life. In addition, during functional unloading, mitochondrial dysfunction is observed and the release of reactive oxygen species (ROS) by mitochondria increases. It is known that some antioxidants can reduce the manifestation of atrophy during functional unloading. We hypothesized that the mitochondria-specific antioxidant Mito-TEMPO would block the accumulation of mitochondrial ROS, prevent the increase in mRNA expression of ubiquitin ligases and the decrease in anabolic parameters during 7 days of functional unloading, which together may reduce the degree of atrophy. In our study, animals receiving Mito-TEMPO during 7 days of hind limb suspension partially prevented the decrease in cross-sectional area of <i>m. soleus</i> muscle fibers, the increase in MuRF-1 and Atrogin mRNA expression, and the decrease in rRNA content. In addition, Mito-TEMPO prevented ROS-dependent tropomyosin oxidation during 7 days of suspension in the soleus muscle. Thus, the accumulation of mitochondrial ROS in the soleus muscle during 7 days of functional unloading affects both protein synthesis and degradation, which is reflected in a decrease in the cross-sectional area of muscle fibers in the rat soleus muscle.</p>

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Antioxidant Mito-TEMPO Partially Prevents Atrophy of the Rat Soleus Muscle after 7 Days of Functional Unloading

  • 期刊论文

  • 发表日期：

    2026年5月20日

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  • D. A. Sidorenko,
  • I. D. Lvova,
  • B. S. Shenkman,
  • K. A. Sharlo

摘要

Abstract

Functional unloading of skeletal muscles is observed during space flight, prolonged bed rest, or limb immobilization. This leads to skeletal muscle atrophy, which has serious health consequences and significantly reduces quality of life. In addition, during functional unloading, mitochondrial dysfunction is observed and the release of reactive oxygen species (ROS) by mitochondria increases. It is known that some antioxidants can reduce the manifestation of atrophy during functional unloading. We hypothesized that the mitochondria-specific antioxidant Mito-TEMPO would block the accumulation of mitochondrial ROS, prevent the increase in mRNA expression of ubiquitin ligases and the decrease in anabolic parameters during 7 days of functional unloading, which together may reduce the degree of atrophy. In our study, animals receiving Mito-TEMPO during 7 days of hind limb suspension partially prevented the decrease in cross-sectional area of m. soleus muscle fibers, the increase in MuRF-1 and Atrogin mRNA expression, and the decrease in rRNA content. In addition, Mito-TEMPO prevented ROS-dependent tropomyosin oxidation during 7 days of suspension in the soleus muscle. Thus, the accumulation of mitochondrial ROS in the soleus muscle during 7 days of functional unloading affects both protein synthesis and degradation, which is reflected in a decrease in the cross-sectional area of muscle fibers in the rat soleus muscle.