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Abstract <p><b>Objective:</b> The enhanced performance of molecular sensors for diagnostics by utilizing a DNA-cassette nanostructure can improve the delivery of fluorogenic substrates to the catalytic core of a binary (split) DNAzyme (BiDz), resulting in reduced background noise compared to traditional designs. <b>Methods:</b> In vitro methods of fluorescence measurement and gel shifts to assess secondary structures, sensitivity and selectivity of the designs were used. <b>Results and Discussion:</b> The modified sensor had reduced background than DNA-cassette-free BiDz sensor of traditional design. <b>Conclusions:</b> This innovation aims to increase the sensitivity and reliability of nucleic acid-based sensors in future in vivo diagnostic applications.</p>

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Substrate Feeding “DNA-Cassette” Improves the Background of Binary (Split) DNAzyme Sensor

  • 期刊论文

  • 发表日期：

    2025年8月19日

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  • Y. S. Kostyuk,
  • M. S. Rubel,
  • T. P. Zemerova,
  • A. A. Rubel

摘要

Abstract

Objective: The enhanced performance of molecular sensors for diagnostics by utilizing a DNA-cassette nanostructure can improve the delivery of fluorogenic substrates to the catalytic core of a binary (split) DNAzyme (BiDz), resulting in reduced background noise compared to traditional designs. Methods: In vitro methods of fluorescence measurement and gel shifts to assess secondary structures, sensitivity and selectivity of the designs were used. Results and Discussion: The modified sensor had reduced background than DNA-cassette-free BiDz sensor of traditional design. Conclusions: This innovation aims to increase the sensitivity and reliability of nucleic acid-based sensors in future in vivo diagnostic applications.