Prospects for the Use of Universal Serum Substitute eBS for Cultivation of Stable Human Cell Lines
摘要
Objective: The transition to culturing continuous cell lines in media based on serum substitutes of various origins has numerous ethical, financial and methodological reasons. Material and methods: The aim of this study was to evaluate the possibility of replacing fetal bovine serum (FBS) with a domestically produced serum substitute eBS. Results and discussion: It was demonstrated that eBS exhibits the main advantages and disadvantages inherent in serum-free media. For melanoma cells mel Il cultured in media supplemented with 10% eBS, without additional introduction of growth and adhesion factors, a transition to a suspension format with reduced cell proliferation was observed. After adaptation, mel Il cells demonstrate the ability to grow in the medium containing 10% eBS only with a minimal additional amount of fetal bovine serum (0.2% FBS). Conclusions: As a cryoprotectant, eBS showed that the viability of mel Il cells was maintained at a level comparable to that of media supplemented with FBS; however, reduced cell survival was observed 24 h after thawing.