Antioxidant Properties of Brown Algae in 3D Model for Colorectal Cancer

A potential scientific viewpoint is provided by three-dimensional (3D) cancer models, which have as their main objective bridging the differences between two-dimensional (2D) models, animal models, and clinical research. We used a tissue engineering approach to engineer colorectal cancer (CT26 cell line) with a decellularized sheep colon to create a 3D biologic model. A decellularized colon matrix (DCM) was prepared with 1% sodium dodecyl sulfate (SDS) and its DNA content, biocompatibility, hemocompatibility, histology, cell adhesion, and tissue ultrastructure were characterized. Brown algae exhibit various biological activities, including anticancer activity, connected to the impact of carotenoids, glyceroglycolipids, fucoidan sulfate polysaccharides, or iodine compounds. In both a 2D culture (culture plate) and 3D (DCM) model, CT26 cells were treated with brown algae extract and doxorubicin (DOX), and their viability, total antioxidant capacity (TAC), and nitric oxide (NO) secretion were assessed. DCM retains a significant amount of its biological and structural characteristics. In both models, cell survival was decreased. Groups that received algae demonstrated antioxidant activity. A decrease in NO secretion was demonstrated in cancer cells that had been treated with algae and DOX, the 3D model’s drug sensitivity was lower than the 2D model. Due to the biological activity of the extracellular matrix, the use of decellularized scaffolds in the construction of cancer models can thus be a potent tool for future research and drug screens.