Isolation of Phytohormone trans-Zeatin: Potential Oxidant Scavenger and Anti-Aging Compound

In all parts of the world, dementia rates are rising rapidly, particularly in Western nations, where it accounts for roughly 60% of cases and is linked to population aging. According to the free radical hypothesis of aging, age-related cellular and tissue damages are brought on by free radicals. Cocos nucifera (L.) has been traditionally utilized in various health conditions, including digestive disorders, antifungal, wound healing, immune support, and cardiovascular health. Objective of the research is to isolate, quantify, and characterize trans-Zeatin (tZn) from hydroalcoholic extract of Cocos nucifera (HECN) and to evaluate its anti-aging and antioxidant potential by studying the Keap-1/HO-1 signaling pathway through in-silico docking analysis. UV, IR, HPLC, and NMR techniques were used for isolation and quantification of tZn. The in vitro anti-oxidant potential of tZn was examined, by using the DPPH, OH, and NO radical scavenging assay. Anti-aging potential of tZn was evaluated by docking analysis against selected proteins (Keap-1 and HO-1) using AutoDock Vina software. From the result it was found that tZn has strong antioxidant potential in a concentration dependent manner with IC₅₀ values of 13.86 ± 2.8, 15.49 ± 3.2, and 16.96 ± 1.5 µg/mL comparable with reference standard ascorbic acid. The molecular docking analysis showed the significant binding potential of the tZn with the active sites of Keap-1 and HO-1, which are crucially involved in the pathophysiology of aging. Based on the conducted studiеs, it is concluded that the tZn from HECN could be measured as a potential anti-oxidant and anti-aging compound.