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Abstract <p>Objective. To study the repertoire of the T-cell receptor in persons chronically exposed to radiation in the long-term period. <b>Materials and methods.</b> The study involved 48 people, who were divided into two groups: a group of exposed persons (31 individuals with the average accumulated dose to red bone marrow (RBM) of 981 ± 130 mGy) and a comparison group (17 individuals with the average accumulated dose to RBM of 25.3 ± 5.91 mGy). The study groups did not differ significantly in age, gender and ethnicity. The repertoire of Vβ-segments of the T-cell receptor of the peripheral blood T-lymphocytes of exposed persons was analyzed by flow cytometry method. 24 Vβ-segments of the T-cell receptor were studied. Statistical processing of the obtained data was carried out using the Wilcoxon signed-rank test, and a direct description of Vβ-segment repertoire of the T-cell receptor was performed using the Lorenz curve and the Gini-TCR index. <b>Results.</b> The study revealed a statistically significant increase in the number of Vβ3 and Vβ5.2 T-cell receptor segments in exposed individuals relative to the comparison group (<i>p</i> = 0.03 and <i>p</i> = 0.003, respectively). It was also shown that the distribution of the Vβ-segments of the T-cell receptor is uneven in both study groups. However, there was no significant difference between the repertoires of the T-cell receptor of the studied groups in the Gini-TCR index (<i>p</i> = 0.14).</p>

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Analysis of Vβ-Segment Diversity of T-Cell Receptor in Residents of the Techa Riverside Villages Chronically Exposed to Radiation in the Long-Term Period

  • 期刊论文

  • 发表日期：

    2025年4月12日

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  • A. I. Kotikova,
  • E. A. Blinova,
  • A. V. Akleyev

摘要

Abstract

Objective. To study the repertoire of the T-cell receptor in persons chronically exposed to radiation in the long-term period. Materials and methods. The study involved 48 people, who were divided into two groups: a group of exposed persons (31 individuals with the average accumulated dose to red bone marrow (RBM) of 981 ± 130 mGy) and a comparison group (17 individuals with the average accumulated dose to RBM of 25.3 ± 5.91 mGy). The study groups did not differ significantly in age, gender and ethnicity. The repertoire of Vβ-segments of the T-cell receptor of the peripheral blood T-lymphocytes of exposed persons was analyzed by flow cytometry method. 24 Vβ-segments of the T-cell receptor were studied. Statistical processing of the obtained data was carried out using the Wilcoxon signed-rank test, and a direct description of Vβ-segment repertoire of the T-cell receptor was performed using the Lorenz curve and the Gini-TCR index. Results. The study revealed a statistically significant increase in the number of Vβ3 and Vβ5.2 T-cell receptor segments in exposed individuals relative to the comparison group (p = 0.03 and p = 0.003, respectively). It was also shown that the distribution of the Vβ-segments of the T-cell receptor is uneven in both study groups. However, there was no significant difference between the repertoires of the T-cell receptor of the studied groups in the Gini-TCR index (p = 0.14).