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Abstract <p><i>Trans-</i>splicing, the process where exons from two different pre-mRNA molecules are joined, is an efficient mechanism for generating diverse isoforms encoded by a single locus. This process has been most thoroughly studied in the <i>mod</i>(<i>mdg</i>4) locus of <i>Drosophila</i>. This locus comprises one gene encoding the constant N-terminal part of the <i>Mod</i>(<i>mdg</i>4) protein and five genes that encode over thirty 3'-exons clustered together, determining the diversity of C-terminal domains. In this study, transgenic lines were obtained where a strong polyadenylation signal from the SV40 virus was inserted into two 3'-exon clusters with the aim of halting transcription and, consequently, reducing the frequency of <i>trans</i>-splicing between the constant exons of the <i>mod</i>(<i>mdg</i>4) gene and the 3'-exons of the modified clusters. Unexpectedly, it was found that SV40 polyadenylation signals had no effect on either the transcription level of the clusters or the efficiency of <i>trans</i>-splicing. The results indicate the ability of RNA polymerase II, transcribing the 3'-exons of the <i>mod</i>(<i>mdg</i>4) locus, to overcome even strong polyadenylation signals from SV40.</p>

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Trans-Splicing Enhances Processivity of RNA Polymerase II and Suppresses Polyadenylation Signals

  • 期刊论文

  • 发表日期：

    2026年2月1日

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  • Iu. V. Soldatova,
  • O. Beginyazova,
  • L. S. Melnikova,
  • A. K. Golovnin,
  • O. G. Maksimenko,
  • P. G. Georgiev,
  • M. V. Tikhonov

摘要

Abstract

Trans-splicing, the process where exons from two different pre-mRNA molecules are joined, is an efficient mechanism for generating diverse isoforms encoded by a single locus. This process has been most thoroughly studied in the mod(mdg4) locus of Drosophila. This locus comprises one gene encoding the constant N-terminal part of the Mod(mdg4) protein and five genes that encode over thirty 3'-exons clustered together, determining the diversity of C-terminal domains. In this study, transgenic lines were obtained where a strong polyadenylation signal from the SV40 virus was inserted into two 3'-exon clusters with the aim of halting transcription and, consequently, reducing the frequency of trans-splicing between the constant exons of the mod(mdg4) gene and the 3'-exons of the modified clusters. Unexpectedly, it was found that SV40 polyadenylation signals had no effect on either the transcription level of the clusters or the efficiency of trans-splicing. The results indicate the ability of RNA polymerase II, transcribing the 3'-exons of the mod(mdg4) locus, to overcome even strong polyadenylation signals from SV40.