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Abstract <p>6-azido-2-chloropurine-2′-deoxyriboside, a valuable precursor for the preparation of modified 2-chloropurine nucleosides substituted at the 6-position of the heterocyclic base, was obtained by enzymatic transglycosylation. 6-azido-2-chloropurine-2′-deoxyriboside can also be used as a photocross-linking agent to study the nucleic acids—proteins interactions. A type II nucleoside deoxyribosyltransferase from <i>Lactobacillus leichmannii</i> was used as a biocatalyst. The optimal conditions for the formation of 6-azido-2-chloropurine-2′-deoxyriboside using 7-methyl-2′-deoxyguanosine as a carbohydrate residue donor were determined.</p>

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Preparation of 6-Azido-2-Chloropurine-2′-Deoxyriboside by Enzymatic Transglycosylation Reaction Catalyzed by Lactobacillus leichmannii Type II Nucleoside Deoxyribosyltransferase

  • 期刊论文

  • 发表日期：

    2025年8月1日

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  • C. S. Alexeev,
  • M. A. Konkina,
  • N. N. Kurochkin,
  • M. S. Drenichev

摘要

Abstract

6-azido-2-chloropurine-2′-deoxyriboside, a valuable precursor for the preparation of modified 2-chloropurine nucleosides substituted at the 6-position of the heterocyclic base, was obtained by enzymatic transglycosylation. 6-azido-2-chloropurine-2′-deoxyriboside can also be used as a photocross-linking agent to study the nucleic acids—proteins interactions. A type II nucleoside deoxyribosyltransferase from Lactobacillus leichmannii was used as a biocatalyst. The optimal conditions for the formation of 6-azido-2-chloropurine-2′-deoxyriboside using 7-methyl-2′-deoxyguanosine as a carbohydrate residue donor were determined.