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Cultivation of Halophilic Archaea Halobacterium salinarum

  • D. D. Kuklina,
  • A. Yu. Shishkin,
  • I. O. Bezruchko,
  • S. V. Kalenov,
  • I. S. Okhrimenko,
  • E. A. Dronova,
  • A. E. Mikhailov,
  • Yu. L. Ryzhykau

摘要

Abstract

A light-sensitive protein from extremophile archaea Halobacterium salinarum, bacteriorhodopsin (HsBR), has found numerous applications in pharmacology, biotechnology, bioelectronics and other fields [1, 2] due to its ability to convert light energy into a gradient of hydrogen ions across the cell membrane. Despite a wide range of its practical applications, the quantum mechanism of proton transfer remains not fully discovered yet. For further investigation and cost-effective implementation of developments, a higher yield of BR-rich biomass is necessitated. Hereafter we present our findings regarding efficient synthesis of HsBR using its natural host, H. salinarum.