Study of the Effect of UV Laser Pulse Duration and Wavelength on Fibroblasts

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发表日期：

2024年8月14日

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Y. M. Hamdan,
D. A. Makarova,
N. I. Shamsutdinov,
P. V. Zelenikhin,
A. S. Nizamutdinov,
A. A. Buglak,
T. A. Telegina

摘要

Abstract

We report the cytotoxic effect of pulse duration and wavelength of UVB laser radiation from Li(Lu,Y)F₄:Ce laser on human skin fibroblasts. When performing the MTT test 24 h after irradiation, an increase in the viability parameter was observed. Flow cytometry showed that 80.8 ± 6.9% of cells have a damaged membrane and only 6.2 ± 2.2% reach the stage of late apoptosis, due to which they have the potential to restore the cytoplasmic membrane and subsequently divide.