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Abstract <p>Menopause increased the vulnerability of women to stress,which affects most of the body tissues, especially the brain andheart. The goal of this study was to explore whether omega-3 fattyacid supplementation can help to reduce stress-related heart problemsin menopausal rats. Female Wistar rats (<i>n</i> =32) were divided into four groups: control, ovariectomy, ovariectomy+ immobilization stress (2 h daily for 2 weeks), and ovariectomy+ immobilization stress (IMO, 2 h daily for 14 consecutive days) plusoral supplementation of omega-3 fatty acids (400 mg/kg BW/d for14 consecutive days). In these groups, we investigated cardiac functionby echocardiography and analysed serum level of IGF-1 and estrogen,in addition to some local cardiac parameters such as troponin-T(cTnT) levels, as well as serum protein levels of lactate dehydrogenase(LDH) and creatine kinase-MB (CKMB). We also measured serum levelsof heat-shock proteins (HSP), atrial natriuretic peptide (ANP),and B-type natriuretic peptide (BNP). The cardiac tissue was alsoexamined histologically and immunohistochemically for iNOS and caspase-3.The data obtained demonstrated a significant change in cardiac weights,abnormal deposition of excess collagen, and dyslipidemia in stressedrats. In addition, we found elevated cardiac CK-MB and LDH activitiesand cTnT levels, together with a reduction of systolic functionof the heart by decreasing EF% and FS%. Importantly, both caspase-3and nitric oxide synthase expression were markedly increased inthe ST-OVX group. Furthermore, stressed rats showed low serum IGF-1and decreased local compensatory peptides like HSP, ANP, and BNP.Omega-3 supplementation improved these parameters; however, theywere still lower than those of control rats. The present study suggests thatthe anti-stress effect of omega-3 supplements is mediated by normalizationof stress-induced changes in the circulating IGF-1 and dyslipidemia.In addition, it possesses an antiapoptotic effect by decreasinglocal expression of caspase-3, thereby returning the body to normalhomeostasis. These data showed that the response to chronic restraintstress-induced cardiac dysfunction was modulated by the omega-3fatty acid supply.</p>

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Protective Effect of Omega-3 Fatty Acid Supplementation against Immobilization Stress-Induced Cardiac Morphological and Functional Abnormalities in the Menopausal Rat Model

  • 期刊论文

  • 发表日期：

    2025年10月1日

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  • Ramadan A. Saad,
  • Reda Ahmed Hasan,
  • Haitham MH Qutob,
  • Howaida S. Ali,
  • Wael M. Elayate,
  • Dalia A. Saad

摘要

Abstract

Menopause increased the vulnerability of women to stress,which affects most of the body tissues, especially the brain andheart. The goal of this study was to explore whether omega-3 fattyacid supplementation can help to reduce stress-related heart problemsin menopausal rats. Female Wistar rats (n =32) were divided into four groups: control, ovariectomy, ovariectomy+ immobilization stress (2 h daily for 2 weeks), and ovariectomy+ immobilization stress (IMO, 2 h daily for 14 consecutive days) plusoral supplementation of omega-3 fatty acids (400 mg/kg BW/d for14 consecutive days). In these groups, we investigated cardiac functionby echocardiography and analysed serum level of IGF-1 and estrogen,in addition to some local cardiac parameters such as troponin-T(cTnT) levels, as well as serum protein levels of lactate dehydrogenase(LDH) and creatine kinase-MB (CKMB). We also measured serum levelsof heat-shock proteins (HSP), atrial natriuretic peptide (ANP),and B-type natriuretic peptide (BNP). The cardiac tissue was alsoexamined histologically and immunohistochemically for iNOS and caspase-3.The data obtained demonstrated a significant change in cardiac weights,abnormal deposition of excess collagen, and dyslipidemia in stressedrats. In addition, we found elevated cardiac CK-MB and LDH activitiesand cTnT levels, together with a reduction of systolic functionof the heart by decreasing EF% and FS%. Importantly, both caspase-3and nitric oxide synthase expression were markedly increased inthe ST-OVX group. Furthermore, stressed rats showed low serum IGF-1and decreased local compensatory peptides like HSP, ANP, and BNP.Omega-3 supplementation improved these parameters; however, theywere still lower than those of control rats. The present study suggests thatthe anti-stress effect of omega-3 supplements is mediated by normalizationof stress-induced changes in the circulating IGF-1 and dyslipidemia.In addition, it possesses an antiapoptotic effect by decreasinglocal expression of caspase-3, thereby returning the body to normalhomeostasis. These data showed that the response to chronic restraintstress-induced cardiac dysfunction was modulated by the omega-3fatty acid supply.