Design, Synthesis, and Urease Inhibition Activities of Some bis-Coumarin-perimidine Hybrid Compounds

A series of bis-coumarin-perimidine hybrids were designed and synthesized, and their structures were characterized by FT-IR, ¹H NMR, ¹³C NMR, and elemental analyses spectral data. All newly synthesized compounds were screened for their urease activity. All synthesized compounds were found to be effective in urease enzyme inhibition. With IC₅₀ values ranging from 0.022 ± 0.50 to 0.098 ± 0.10 µM, the compounds exhibit more active inhibition than the standard thiourea (IC₅₀ = 0.206 ± 0.25 µM). The compound N′,N′′-(2,2′-(2,2′-(2-oxo-1H-perimidine-1,3(2H)-diyl)bis(acetyl))bis(6-bromo-2-oxo-2H-chromene-3-carbohydrazide) has the best urease inhibition with IC₅₀ = 0.022 ± 0.50 µM.