Abstract <p>Silver nanoparticles with enhanced antibacterial activity were fabricated by green reduction method with tannic acid for utilizing the synergistic antibacterial effect of tannic acid and silver nanoparticles. The chemical composition and morphology of the tannic acid-stabilized silver nanoparticle were characterized by using Fourier-transform infrared spectroscopy, X-ray diffraction, scanning electron and transmission electron microscopy. The optimal preparation conditions for tannic acid-stabilized silver nanoparticle were the molar ratio of tannic acid solution to AgNO₃ of 1 : 4, the pH value of tannic acid solution of 10, the reaction time of 30 min, and the reaction temperature of 60°C. The prepared tannic acid-stabilized silver nanoparticle showed excellent antibacterial effects against <i>Escherichia coli, Staphylococcus aureus,</i> and <i>Pseudomonas aeruginosa</i>.</p>